PXD012289

PXD012289 is an original dataset announced via ProteomeXchange.

Title	Dynamic Bovine Milk Proteome Alterations during Staphylococcus aureus Infection in Subclinical and Clinical Mastitis
Description	Bovine mastitis, predominantly caused by pathogenic Staphylococcus aureus, is a major impediment to milk production. Although its prevalence varies from cows to buffaloes, it lacks relevant markers that mark the phases and progression of the disease. Thus, the focus of this study was to identify key proteins that mark the transition from subclinical to clinical states in a species specific manner. Whey proteins isolated from healthy and S. aureus infected milk from both Holstein Friesian cow and Murrah buffalo were characterized by label free quantification using mass spectrometry. Collectively, 662 proteins were identified with atleast two peptides, of which 51 and 80 proteins were found significant by ANOVA (p value < 0.05) with increasing or decreasing trends in the disease progression in HF and Mu, respectively. Linear change in expression of proteins like haptoglobin and fibronectin in HF and spermadhesin and osteopontin in Mu correlated with the stage of the disease and progression in a species specific manner. Similarly, high mobility group proteins, angiogenin, cofilin-1 that were previously reported in oxidative stress were overexpressed whilemembers of ubiquitin family which remove aggregated proteins were downregulated during the progression of infection. Subsequently, selected differentially expressed proteins (e.g. osteopontin, CAP-1 and fibrinogen α) were validated by Western blot analysis. Results of this study provide deeper insights on whey proteome dynamics and a signature pattern indicative of disease progression.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:05:47.727.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sudipa Maity
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-09 07:46:03	ID requested
⏵ 1	2022-02-22 04:05:48	announced

Maity S, Rubi, ć I, Kule, š J, Horvati, ć A, Đ, uri, č, i, ć D, Samard, ž, ija M, Ljubi, ć BB, Turk R, Gra, č, ner D, Ma, ć, e, š, i, ć N, Valpoti, ć H, Mrljak V, Integrated Metabolomics and Proteomics Dynamics of Serum Samples Reveals Dietary Zeolite Clinoptilolite Supplementation Restores Energy Balance in High Yielding Dairy Cows. Metabolites, 11(12):(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: Bovine Whey/ Subclinical Mastitis/ Clinical Mastitis/ Label free quantification

Kiran Ambatipudi
contact affiliation	#*Assistant Professor Department of Biotechnology Indian Institute of Technology Roorkee
contact email	kiranfbt@iitr.ac.in
lab head
Sudipa Maity
contact affiliation	Indian Institute of Technology Roorkee
contact email	sudipabiotech.iitr@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD012289

PRIDE project URI

• PRIDE
  1. PXD012289
     1. Label: PRIDE project
     2. Name: Dynamic Bovine Milk Proteome Alterations during Staphylococcus aureus Infection in Subclinical and Clinical Mastitis