PXD012283 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | serum exosomes contain SPARC and LRG1 as biomarkers for colon cancer and differ by tumor primary location |
Description | Purpose: Over the past few years, the distinction between left- and right-sided colon cancer has been brought into focus. Right-sided tumor location was associated with an inferior overall survival and progression-free survival. We aimed to perform a detailed analysis of the diversity in exosomes between left- and right-sided colon cancer using quantitative proteomics. Experimental Design: We isolated exosomes from left- and right-sided colon cancer patients and healthy volunteers and treated colorectal cancer cell line with serum-derived exosomes. Then we performed quantitative proteomics analysis of the serum-derived exosomes and cell line treated with exosomes, respectively. Results: The expression profile of the serum exosome proteome in patients with right-sided colon cancer is different from patients with left-sided colon cancer. Serum-derived exosomes of right-sided colon cancer promote metastasis via up-regulation of extracellular matrix-related proteins, especially proteoglycans like SPARC and glycoprotein like LRG1. Exosomal SPARC and LRG1 were closely correlated with progression-free survival. Conclusions: Proteomic analysis identified different exosomal protein profiling between left- and right-sided colon cancer. Serum-derived exosomes of right-sided colon cancer promote metastasis via overexpression of SPARC and LRG1. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:52:07.924.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Ge |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-09 02:33:52 | ID requested | |
1 | 2019-11-25 03:49:12 | announced | |
⏵ 2 | 2024-10-22 04:52:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.ebiom.2019.11.003; |
Zhong ME, Chen Y, Xiao Y, Xu L, Zhang G, Lu J, Qiu H, Ge W, Wu B, Serum extracellular vesicles contain SPARC and LRG1 as biomarkers of colon cancer and differ by tumour primary location. EBioMedicine, 50():211-223(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: exosome, proteomics,colon cancer, LRG1, SPARC, location, metastasis |
Contact List
Wei Ge |
contact affiliation | National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences |
contact email | wei.ge@chem.ox.ac.uk |
lab head | |
Wei Ge |
contact affiliation | National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences |
contact email | wei.ge@chem.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012283
- Label: PRIDE project
- Name: serum exosomes contain SPARC and LRG1 as biomarkers for colon cancer and differ by tumor primary location