PXD012280

PXD012280 is an original dataset announced via ProteomeXchange.

Title	Delineation of the olive pollen proteome: A focus on its allergen families reveals cyclophilin as a relevant allergen
Description	The delineation of the olive pollen proteome and its allergogram can improve the clinical management of patients with this pollinosis. We here integrated the recently described wild olive genomic data in a comprehensive proteomic approach to get the annotated olive (Olea europaea) pollen proteome and complete its complex allergogram. Olive pollen proteins were identified by LC-MS/MS using predicted protein sequences from its genome. GO annotation, KEGG Pathway analysis and identification of allergen families were performed by bioinformatics. Recombinant DNA, protein expression and purification, and immunological analyses were used to characterize putative allergens. A total of 1,907 proteins were identified. 60% of the proteins were predicted to possess catalytic activity and be involved in metabolic processes. 203 proteins belonging to 47 allergen families were found, with 37 non-previously described in olive pollen. Of four potential allergens produced in Escherichia coli, a peptidyl-prolyl cis-trans isomerase -cyclophilin-, masked in the protein extract by the major allergen Ole e 1, was found as a new olive pollen allergen (Ole e 15). 63% of the Ole e 15-sensitized patients were children and showed strong IgE recognition of the allergen. Ole e 15 shared high sequence identity with other plant, animal and fungal cyclophilins and a high IgE cross-reactivity with pollen, plant food and animal extracts. Taken together, the combination of available genomic data with proteomics permitted the profiling of the olive pollen proteome, revealing the spectrum of allergen families and cyclophilin as a new relevant allergen implicated in cross-reactivity.
HostingRepository	PRIDE
AnnounceDate	2019-06-17
AnnouncementXML	Submission_2019-06-17_04:05:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rodrigo Barderas
SpeciesList	scientific name: Olea europaea (Common olive); NCBI TaxID: 4146;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-09 02:14:09	ID requested
⏵ 1	2019-06-17 04:05:34	announced

San Segundo-Acosta P, Oeo-Santos C, Bened, é S, de Los R, í, os V, Navas A, Ruiz-Leon B, Moreno C, Pastor-Vargas C, Jurado A, Villalba M, Barderas R, Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen. J Proteome Res, 18(8):3052-3066(2019) [pubmed]

curator keyword: Biological

submitter keyword: olive pollen proteome, pollinosis, olive pollen allergens, proteomics

Rodrigo Barderas
contact affiliation	UFIEC, Chronic Disease Programme, Functional Proteomics Unit, Instituto de Salud Carlos III
contact email	r.barderasm@isciii.es
lab head
Rodrigo Barderas
contact affiliation	Instituto de Salud Carlos III
contact email	r.barderasm@isciii.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012280
     1. Label: PRIDE project
     2. Name: Delineation of the olive pollen proteome: A focus on its allergen families reveals cyclophilin as a relevant allergen