PXD012259

PXD012259 is an original dataset announced via ProteomeXchange.

Title	Comparative Phosphoproteomic Profiling of Type III Adenylyl Cyclase Knockout and Control, Male and Female Mice Prefrontal Cortex
Description	Type III adenylyl cyclase (AC3, ADCY3) is predominantly enriched in neuronal primary cilia throughout the central nervous system (CNS). Genome-wide association studies in humans have associated ADCY3 with major depressive disorder and autistic spectrum disorder, both of which exhibit sexual dimorphism. To date, it is unclear how AC3 affects protein phosphorylation and signal networks in central neurons, and what causes the sexual dimorphism of autism. We employed a mass spectrometry (MS)-based phosphoproteomic approach to quantitatively profile differences in phosphorylation between inducible AC3 knockout (KO) and wild type (WT), male and female mice. In total, we identified 4655 phosphopeptides from 1756 proteins, among which 565 phosphopeptides from 322 proteins were repetitively detected in all samples. Comparison of AC3 KO and WT datasets revealed that phosphopeptides with motifs matching proline-directed kinases’ recognition sites had a lower abundance in the KO dataset than in WTs. We detected 14 phosphopeptides restricted to WT dataset (i.e., from Spast and Ppp1r14a) and 35 exclusively in KOs (i.e., from Sptan1, Arhgap20, Arhgap44, and Pde1b). Moreover, 95 phosphopeptides were identified only in female dataset and 26 only in males. Label-free MS spectrum quantification using Skyline further identified phosphopeptides that had high expression abundance in each sample group. In total, over 200 modifications were gender-biased and had increased expression in females relative to males. 29% gender-biased phosphopeptides were from autism-associated proteins, including Dlg1, Dlg4, Dlgap2, Syn1, Syngap1, Ctnna1, Ctnnd1, Ctnnd2, Pkp4, and Arvcf. These autism proteins were well-connected in protein-protein interaction network that centered around Dlg4. This study provided the first phosphoproteomics evidence, suggesting that gender-biased post-translational phosphorylation may be implicated in the sexual dimorphism of autism.
HostingRepository	PRIDE
AnnounceDate	2019-04-10
AnnouncementXML	Submission_2019-04-10_11:58:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuxin Zhou
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-08 00:14:20	ID requested
⏵ 1	2019-04-10 11:58:37	announced

Zhou Y, Qiu L, Sterpka A, Wang H, Chu F, Chen X, Comparative Phosphoproteomic Profiling of Type III Adenylyl Cyclase Knockout and Control, Male, and Female Mice. Front Cell Neurosci, 13():34(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, Phosphoproteomics, Adcy3, Gender-Biased

Xuanmao Chen
contact affiliation	University of New Hampshire
contact email	xuanmao.chen@unh.edu
lab head
Yuxin Zhou
contact affiliation	University of New Hampshire
contact email	yz1037@wildcats.unh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012259
     1. Label: PRIDE project
     2. Name: Comparative Phosphoproteomic Profiling of Type III Adenylyl Cyclase Knockout and Control, Male and Female Mice Prefrontal Cortex