PXD012252

PXD012252 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis reveals that Odoroside A triggers G2/M arrest and apoptosis in colorectal carcinoma through ROS-p53 pathway
Description	Colorectal cancer (CRC) is one of the most common cancers worldwide with poor survival and limited therapeutic options. There is an urgent need for developing novel therapeutic agents against CRC with good treatment efficiency and low toxicity. Odoroside A (OA) is an active compound isolated from the leaves of Nerium oleander Linn. (Apocynaceae). This study aims to examine the anticancer bioactivity of OA against CRC cells and to investigate the action mechanisms involved. As a result, OA was found to induce apoptosis and exert a significant inhibitory effect on the proliferation and colony-forming ability of CRC cells in a dose-dependent manner without any significant cytotoxicity in normal colorectal epithelial cells. Mechanistically, quantitative proteomics coupled with bioinformatics was performed to explore the OA-regulated proteins and signaling pathways. As suggested by the proteomic analysis, Western blot analysis and flow cytometry validated that exposure of CRC cells to OA caused cell cycle arrest and apoptosis, accompanied with the activation of the ROS/p53 signaling pathway. This observation demonstrated that OA, as a natural product, can induce ROS to suppress tumor cell growth, implicating a novel therapeutic agent against CRC without obvious side effects.
HostingRepository	iProX
AnnounceDate	2019-01-09
AnnouncementXML	Submission_2019-12-10_22:34:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanyan Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-07 23:39:30	ID requested
1	2019-01-07 23:45:41	announced
2	2019-01-09 19:15:18	announced	Update contact name.
⏵ 3	2019-12-10 22:34:04	announced	2019-01-10: Update contact name. 2019-01-10: Update contact information. 2019-12-11: Update publication information.

Chen YY, Wen SY, Deng CM, Yin XF, Sun ZH, Jiang MM, He QY, Proteomic Analysis Reveals that Odoroside A Triggers G2/M Arrest and Apoptosis in Colorectal Carcinoma Through ROS-p53 Pathway. Proteomics, 19(15):e1900092(2019) [pubmed]

submitter keyword: Odoroside A, Natural product, Colorectal cancer, Cell cycle, Apoptosis, ROS/p53 pathways

Qingyu He
contact affiliation	Jinan university
contact email	tqyhe@email.jnu.edu.cn
lab head
Yanyan Chen
contact affiliation	Jinan university
contact email	yanyanchan@126.com
dataset submitter

iProX dataset URI