PXD012250

PXD012250 is an original dataset announced via ProteomeXchange.

Title	Quantitative assay of targeted proteome in trichome glandular cells using large-scale selected reaction monitoring strategy
Description	Background: Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in the glandular cell. In order to systematically understand the metabolic processes in the glandular cells, it is indispensable to analyze the cellular proteome dynamics. The conventional proteomics methods based on mass spectrometry have been enabled large-scale protein analysis, but requires a large number of trichome samples for in-depth analysis and is not suitable for rapid and sensitive quantification of targeted proteins. Results: Here we present a high-throughput strategy for quantifying targeted proteins in a specific trichome glandular cell, using selected reaction monitoring (SRM) assay. SRM assay platform, targeting the proteins in tomato type VI trichome gland cells as a model study, demonstrated its effectiveness in multiple protein quantitation from a limited sample amount. A large-scale SRM assay developed using triple quadrupole mass spectrometer online connected to nanoflow liquid chromatography has enough capability to accurately measure the expression level of 221 targeted proteins contained in the glandular cell sample recovered from the 100 glandular trichomes within 120 minutes. Conclusions: Targeted proteomics approach using established SRM assays makes it possible to quantify the proteins of interest with minimum effort in the sampling. The remarkable success of the SRM assay developments by a simple experimental workflow will increase the plant biologist's accessibility to proteomics research for glandular trichome.
HostingRepository	PanoramaPublic
AnnounceDate	2019-08-13
AnnouncementXML	Submission_2019-08-13_14:59:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nobuaki Takemori
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	Propionamide
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-07 21:14:59	ID requested
⏵ 1	2019-08-13 14:59:01	announced

Takemori A, Nakashima T, Ô, mura H, Tanaka Y, Nakata K, Nonami H, Takemori N, Quantitative assay of targeted proteome in tomato trichome glandular cells using a large-scale selected reaction monitoring strategy. Plant Methods, 15():40(2019) [pubmed]

submitter keyword: Tomato type VI trichome glandular cell

Nobuaki Takemori
contact affiliation	Ehime University
contact email	takemori@m.ehime-u.ac.jp
lab head
Nobuaki Takemori
contact affiliation	Ehime University
contact email	takemori@m.ehime-u.ac.jp
dataset submitter

Panorama Public dataset URI