PXD012245 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Label-free, quantitative analysis of PKA-dependent phosphoproteome changes in Trypanosoma brucei |
Description | Protein kinase A (PKA), the main effector of second messenger cAMP, is highly conserved among eukaryotes and a paradigm for the mechanisms of regulation of protein kinases by ligands. The unique PKA holoenzymes in the phylogenetically distant protozoan parasite Trypanosoma are unresponsive to cAMP in vitro and in vivo. By small molecule screening and optimization, we designed direct, membrane-permeable activators binding with a kD of 9 nM to the CNB pockets of the T. brucei regulatory PKA subunit. 7-Cyano-7-deazainosine has low toxicity and thus is a perfect tool to explore cAMP-independent PKA signaling in these important pathogens. This project describes PKA-induced phosphoproteome changes of the bloodstream stage of T. brucei. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-25 |
AnnouncementXML | Submission_2019-04-03_04:36:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignasi Forne |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-07 08:28:53 | ID requested | |
1 | 2019-02-25 05:15:43 | announced | |
⏵ 2 | 2019-04-03 04:36:53 | announced | Updated publication reference for PubMed record(s): 30926779. |
Publication List
Bachmaier S, Volpato Santos Y, Kramer S, Githure GB, Kl, ö, ckner T, Pepperl J, Baums C, Schenk R, Schwede F, Genieser HG, Dupuy JW, Forn, é I, Imhof A, Basquin J, Lorentzen E, Boshart M, Nucleoside analogue activators of cyclic AMP-independent protein kinase A of Trypanosoma. Nat Commun, 10(1):1421(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Protein kinase A |
PKA |
Trypanosoma |
7-Cyano-7-deazainosine |
label-free quantitative phosphoproteome analysis |
Contact List
Prof. Dr. Michael Boshart (Genetics, Biochemistry, Cell Biology) Prof. Dr. Michael Boshart |
contact affiliation | Biozentrum LMU, Genetics Großhaderner Strasse 2-4 82152 Planegg-Martinsried phone +4989218074603 http://www.genetik.biologie.uni-muenchen.de/research/boshart/index.html |
contact email | boshart@lrz.uni-muenchen.de |
lab head | |
Ignasi Forne |
contact affiliation | Biomedical Center-LMU |
contact email | ignasi.forne@lrz.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012245
- Label: PRIDE project
- Name: Label-free, quantitative analysis of PKA-dependent phosphoproteome changes in Trypanosoma brucei