PXD012242

PXD012242 is an original dataset announced via ProteomeXchange.

Title	Proteogenomics uncovers critical elements of host response in bovine soft palate epithelial cells following in vitro infection with foot-and-mouth disease virus (FMDV)
Description	: Foot-and-mouth disease (FMD) is the most devastating disease of cloven-hoofed livestock, with a crippling economic burden in endemic areas and immense costs associated with outbreaks in free countries. Foot-and-mouth disease virus (FMDV), a picornavirus, will spread rapidly in naïve populations, reaching morbidity rates of up to 100% in cattle. Even after recovery, over 50% of cattle remain subclinically infected and infectious virus can be recovered from the nasopharynx. The pathogen and host factors that contribute to FMDV persistence are currently not understood. Using for the first time primary bovine soft palate multilayers in combination with proteogenomics, we analyzed the transcriptional responses during acute and persistent FMDV infection. During the acute phase viral RNA and protein was detectable in large quantities and in response hundreds of interferon-stimulated genes (ISG) were overexpressed, mediating antiviral activity and apoptosis. Although the number of pro-apoptotic ISGs and the extent of their regulation decreased during persistence, some ISGs with antiviral activity were still highly expressed at that stage. This indicates a long-lasting but ultimately ineffective stimulation of ISGs during FMDV persistence. Furthermore, downregulation of relevant genes suggests an interference with the extracellular matrix that may contribute to the skewed virus-host equilibrium in soft palate epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2019-03-06
AnnouncementXML	Submission_2019-03-06_02:12:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-07 02:25:42	ID requested
1	2019-01-17 04:42:52	announced
2	2019-01-18 00:31:39	announced	Updated project metadata.
⏵ 3	2019-03-06 02:12:31	announced	Updated project metadata.

Pfaff F, H, ä, gglund S, Zoli M, Blaise-Boisseau S, Laloy E, Koethe S, Z, ü, hlke D, Riedel K, Zientara S, Bakkali-Kassimi L, Valarcher JF, H, ö, per D, Beer M, Eschbaumer M, Proteogenomics Uncovers Critical Elements of Host Response in Bovine Soft Palate Epithelial Cells Following In Vitro Infection with Foot-And-Mouth Disease Virus. Viruses, 11(1):(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Foot-and-mouth disease virus (FMDV)

bovine soft palate

nasopharynx

transcriptomics

proteomics

bioinformatics

virus-host interaction

innate immune system

interferon-stimulated genes (ISG)

Katharina Riedel
contact affiliation	University of Greifswald; Institute of Microbiology
contact email	riedela@unbi-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012242
     1. Label: PRIDE project
     2. Name: Proteogenomics uncovers critical elements of host response in bovine soft palate epithelial cells following in vitro infection with foot-and-mouth disease virus (FMDV)