PXD012241

PXD012241 is an original dataset announced via ProteomeXchange.

Title	The role of the Ser/Thr kinase PrkC of Clostridium difficile in cell wall homeostasis and antimicrobial resistance.
Description	Clostridium difficile is the leading cause of antibiotic associated diarrhea in adults. During infection, the bacteria must rapidly respond and adapt to the host environment by being able to activate survival strategies. Ser/Thr kinases (STKs) are involved in signal transduction and regulate numerous physiological processes. PrkC of C. difficile is STK with two PASTA domains. We showed that this protein was membrane associated and localized at the septum. We observed that prkC deletion affects the cell morphology with an increased mean size, heterogeneity in length and the presence of abnormal septa. A ∆prkC mutant was able to sporulate and germinate but had a reduced motility and formed more biofilms than the wild-type strain. Moreover, a ∆prkC mutant showed an increased sensitivity to antimicrobial compounds targeting the envelope such as deoxycholate, a secondary bile salt, cephalosporins acting on peptidoglycan synthesis, cationic antimicrobial peptides and lysozyme. This increased susceptibility was not associated to differences in the structure of peptidoglycan or of polysaccharide II (PSII). A proteomic study showed that the majority of C. difficile proteins associated to the cell wall are less abundant in the ∆prkC mutant compared to the WT strain. Finally, the ∆prkC mutant presented a delay in gut establishment in a hamster model of infection while its virulence was not significantly affected.
HostingRepository	PRIDE
AnnounceDate	2019-06-14
AnnouncementXML	Submission_2019-06-14_00:34:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Clostridioides difficile; NCBI TaxID: 1496;
ModificationList	L-methionine sulfoxide; N-acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-07 01:46:09	ID requested
⏵ 1	2019-06-14 00:34:33	announced

Cuenot E, Garcia-Garcia T, Douche T, Gorgette O, Courtin P, Denis-Quanquin S, Hoys S, Tremblay YDN, Matondo M, Chapot-Chartier MP, Janoir C, Dupuy B, Candela T, Martin-Verstraete I, The Ser/Thr Kinase PrkC Participates in Cell Wall Homeostasis and Antimicrobial Resistance in Clostridium difficile. Infect Immun, 87(8):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Clostridium difficile, cell wall, Shotgun proteomics, QExactive HF

Bruno Dupuy
contact affiliation	Laboratoire Pathogénese des Bactéries Anaérobies, InstitutPasteur, Paris, France
contact email	bruno.dupuy@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012241
     1. Label: PRIDE project
     2. Name: The role of the Ser/Thr kinase PrkC of Clostridium difficile in cell wall homeostasis and antimicrobial resistance.