PXD012235 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Efficient checkpoint signaling depends on the integrated activities of Bub1 and the RZZ complex |
Description | Kinetochore localized Mad1 is essential for generating a “wait anaphase” signal during mitosis hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zwilch-ZW10 (RZZ) complex exist. By combining genome editing and RNAi we achieve penetrant removal of Bub1 and Rod in human cells, which reveals that efficient checkpoint signaling depends on the integrated activities of these proteins. Rod removal reduces the proximity of Bub1 and Mad1 and we can bypass the requirement for Rod by tethering Mad1 to kinetochores or increasing the strength of the Bub1-Mad1 interaction. We find that Bub1 has checkpoint functions independent of Mad1 localization that are supported by low levels of Bub1 suggesting a catalytic function. In conclusion, our results support an integrated model for the Mad1 receptors in which the primary role of RZZ is to localize Mad1 at kinetochores to generate the Mad1-Bub1 complex. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:52:05.382.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fabian Coscia |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-01-07 01:34:58 | ID requested | |
1 | 2019-02-19 08:03:36 | announced | |
2 | 2019-05-27 01:41:19 | announced | Updated publication reference for PubMed record(s): 30782962. |
⏵ 3 | 2024-10-22 04:52:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
Zhang G, Kruse T, Guasch Bold, ú C, Garvanska DH, Coscia F, Mann M, Barisic M, Nilsson J, Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex. EMBO J, 38(7):(2019) [pubmed] |
10.15252/embj.2018100977; |
Keyword List
curator keyword: Biological |
submitter keyword: Mitosis Proteomics BUB1 |
Contact List
Jakob Nilsson |
contact affiliation | Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark |
contact email | jakob.nilsson@cpr.ku.dk |
lab head | |
Fabian Coscia |
contact affiliation | Max Delbrueck Center for Molecular Medicine |
contact email | fabian.coscia@cpr.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012235
- Label: PRIDE project
- Name: Efficient checkpoint signaling depends on the integrated activities of Bub1 and the RZZ complex