PXD012231

PXD012231 is an original dataset announced via ProteomeXchange.

Title	Buffalo testis ubiquitome LC-MS/MS
Description	Protein ubiquitination, a major and conserved post-translational modification, is known to play a critical regulatory role in many biological processes in eukaryotes. Although several ubiquitinated proteins have been found in buffalo (Bubalus bubalis) testis in our previous studies, large-scale profiling of buffalo testis ubiquitome has not been reported to date. In this study, we firstly identified a global profiling of lysine ubiquitination of adult buffalo testis using a highly sensitive LC-MS/MS coupled with immune-affinity enrichment of ubiquitinated peptides. In total, 422 lysine ubiquitination sites were identified in 262 proteins in adult buffalo testis tissue. Bioinformatic analysis showed that the ubiquitinated proteins are involved in a variety of biological processes and diverse subcellular localizations. Besides, KEGG pathway and protein interaction network analysis indicated that proteasome, glycolysis/gluconeogenesis and gap junction pathways are modulated by protein ubiquitination in testis. Taken together, these data provide a global view of ubiquitome in buffalo testis for the first time, and serve as an important resource for exploring the physiological role of lysine ubiquitination in testis in mammalian.
HostingRepository	PRIDE
AnnounceDate	2020-06-05
AnnouncementXML	Submission_2020-06-05_03:17:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu-lin Huang
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-03 03:53:15	ID requested
⏵ 1	2020-06-05 03:17:30	announced

Huang YL, Zhang PF, Hou Z, Fu Q, Li MX, Huang DL, Deng TX, Lu YQ, Liang XW, Zhang M, Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis. Biosci Rep, 40(6):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Adult buffalo, testis, lysine ubiquitination, proteome, LC-MS/MS

ming zhang
contact affiliation	State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning 530004, Guangxi, PR China.
contact email	mingzhang@gxu.edu.cn
lab head
Yu-lin Huang
contact affiliation	Guangxi University of Chinese Medicine
contact email	huangyulin1016@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012231
     1. Label: PRIDE project
     2. Name: Buffalo testis ubiquitome LC-MS/MS