PXD012227

PXD012227 is an original dataset announced via ProteomeXchange.

Title	PhDHS is involved in chloroplast development in petunia
Description	Deoxyhypusine synthase (DHS) is encoded by a nuclear gene and is the key enzyme involved in the post-translational activation of the eukaryotic translation initiation factor eIF5A. DHS plays important roles in plant growth and development. To gain a better understanding of DHS, the petunia (Petunia hybrida) PhDHS gene was isolated, and the role of PhDHS in plant growth was analysed. PhDHS protein was localized to the nucleus and cytoplasm. Virus-mediated PhDHS silencing caused a sectored chlorotic leaf phenotype. The chlorophyll levels was reducted and the development of chloroplasts was abnormal in PhDHS-silenced leaves These features were not observed in DHS-suppressed Arabidopsis thaliana and Solanum lycopersicum. These results indicated that PhDHS is required for development in petunia. A proteome assay showed that 308 proteins are up-regulated and 266 proteins are down-regulated in PhDHS-silenced plants compared with control. Among them, 20 proteins in photosystem I and photosystem II and 11 thylakoid proteins were down-regulated in PhDHS-silenced leaves, further supporting the involvement of PhDHS in photosynthesis in petunia.
HostingRepository	PRIDE
AnnounceDate	2019-11-13
AnnouncementXML	Submission_2019-11-12_16:57:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaomei li
SpeciesList	scientific name: Petunia; NCBI TaxID: 4101;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-03 03:52:37	ID requested
⏵ 1	2019-11-12 16:57:12	announced

Liu J, Chang X, Ding B, Zhong S, Peng L, Wei Q, Meng J, Yu Y, PhDHS Is Involved in Chloroplast Development in Petunia. Front Plant Sci, 10():284(2019) [pubmed]

curator keyword: Biological

submitter keyword: Deoxyhypusine synthase, Chloroplast development, sectored chlorotic leaf, Photosynthesis, Petunia

Yixun Yu
contact affiliation	Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	yuyixun@scau.edu.cn
lab head
xiaomei li
contact affiliation	PTM Biolabs lnc.
contact email	xiaomei_li@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012227
     1. Label: PRIDE project
     2. Name: PhDHS is involved in chloroplast development in petunia