PXD012221

PXD012221 is an original dataset announced via ProteomeXchange.

Title	Alterations of human plasma proteome profile on adaptation to high-altitude hypobaric hypoxia
Description	For individuals migrating to or residing permanently at high-altitude regions, environmental hypobaric hypoxia is a primary challenge which induces several physiological or pathological responses. It is well documented that human beings adapt to hypobaric hypoxia via some protective mechanisms, such as erythropoiesis and overproduction of hemoglobin, however little is known on the changes of plasma proteome profiles in accommodation to high-altitude hypobaric hypoxia. In the present study, we investigated differential plasma proteomes of high altitude natives and lowland normal controls by a TMT-based proteomic approach. A total of 818 proteins were identified, of which 137 were differentially altered. Bioinformatics (including GO, KEGG, protein-protein interactions, etc.) analysis revealed the dysregulated proteins were primarily involved in complement and coagulation cascades, anti-oxidative stress and glycolysis. Validations via magnetic Luminex® Assays and ELISA demonstrated that CCL18, C9, PF4, MPO and S100A9 notably up-regulated, and HRG and F11 down-regulated in high altitude natives compared with lowland controls, which were consistent with the proteomic results. Our findings highlight the roles of complement and coagulation cascades, anti-oxidative stress and glycolysis in acclimatization to hypobaric hypoxia and provide a foundation for developing potential diagnostic or/and therapeutic biomarkers for high altitude hypobaric hypoxia-induced diseases.
HostingRepository	PRIDE
AnnounceDate	2019-03-27
AnnouncementXML	Submission_2019-03-27_05:37:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaonan fu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-02 07:28:15	ID requested
⏵ 1	2019-03-27 05:37:19	announced

Du X, Zhang R, Ye S, Liu F, Jiang P, Yu X, Xu J, Ma L, Cao H, Shen Y, Lin F, Wang Z, Li C, Alterations of Human Plasma Proteome Profile on Adaptation to High-Altitude Hypobaric Hypoxia. J Proteome Res, 18(5):2021-2031(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: proteomics

high altitude

hypobaric hypoxia

complement and coagulation cascades

antioxidant

glycolysis.

Zongkui Wang
contact affiliation	Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, China, 610052
contact email	zongkui.wang@ibt.pumc.edu.cn
lab head
xiaonan fu
contact affiliation	zhejiang scientific technology universitiy
contact email	1477953185@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012221
     1. Label: PRIDE project
     2. Name: Alterations of human plasma proteome profile on adaptation to high-altitude hypobaric hypoxia