PXD012219

PXD012219 is an original dataset announced via ProteomeXchange.

Title	Human serum glycopeptide LC-MS/MS
Description	The heterogeneity caused by post-translational modifications and wide dynamic range of relative protein abundances of the human serum proteome, challenge the capabilities of existing mass spectrometry-based methodologies in accurate identifying N-linked glycopeptides from human serum. To overcome these challenges, we utilized pParse 2.0 to find monoisotopic peak of each precursor ion as well as co-eluted ones, then applied spectral library search to intact glycopeptide identification through pMatchGlyco. Spectra of deglycosylated peptide including semi-tryptic peptides and common modifications were used for spectral library construction. Through scoring of ion matches on MS/MS spectra and target-decoy false positive rate control, and manual check of co-eluted precursor ions at MS1 level, the accuracy of identification was improved. In total, this method identified 1,194 N-linked glycosites and 448 unique glycoproteins in human serum.
HostingRepository	PRIDE
AnnounceDate	2019-01-07
AnnouncementXML	Submission_2019-01-07_08:26:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lian Shu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-02 07:27:47	ID requested
⏵ 1	2019-01-07 08:26:39	announced

Dataset with its publication pending

curator keyword: Technical

submitter keyword: Human,serum glycopeptide

Fuquan Yang; Yan Fu
contact affiliation	Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;
contact email	fqyang@ibp.ac.cn
lab head
Lian Shu
contact affiliation	Institute of Biophysics,Chinese Academy of Sciences
contact email	shulian13@mails.ucas.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD012219

PRIDE project URI

• PRIDE
  1. PXD012219
     1. Label: PRIDE project
     2. Name: Human serum glycopeptide LC-MS/MS