PXD012217

PXD012217 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry identification and systems analysis of cytosolic protein N- lysine methylation in anucleate blood platelets
Description	Here, we characterize the methyl-lysine (meK) proteome of anucleate blood platelets. With high-resolution, multiplex mass spectrometry methods, we identify 190 mono-, di- and tri-meK modifications on 150 different platelet proteins, including 28 quantifiable meK modifications consistently present in platelets from multiple individuals. In addition to identifying meK modifications on calmodulin (CaM), GRP78 and EF1A1 that have been previously characterized in other cell types, we also uncover more novel modifications on numerous other cytosolic proteins. Together, our results and analyses support roles for lysine methylation in mediating cytoskeletal, translational, secretory and other cytosolic cellular processes.
HostingRepository	PRIDE
AnnounceDate	2019-04-18
AnnouncementXML	Submission_2019-04-18_02:23:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monomethylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-02 05:20:52	ID requested
⏵ 1	2019-04-18 02:23:30	announced

Rocheleau AD, Melrose AR, Cunliffe JM, Klimek J, Babur Ö, Tassi Yunga S, Ngo ATP, Pang J, David LL, McCarty OJT, Aslan JE, Lysine Methylation in Anucleate Blood Platelets. Proteomics, 19(11):e1900001(2019) [pubmed]

curator keyword: Biological

submitter keyword: isobaric labeling, tandem mass tags, lysine methylation

methyllysine

methylation

platelets

post-translational modifications

Dr. Joseph E. Aslan
contact affiliation	Knight Cardiovascular Institute Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA
contact email	aslanj@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012217

PRIDE project URI

• PRIDE
  1. PXD012217
     1. Label: PRIDE project
     2. Name: Mass spectrometry identification and systems analysis of cytosolic protein N- lysine methylation in anucleate blood platelets