PXD012193

PXD012193 is an original dataset announced via ProteomeXchange.

Title	Glomerular filtrate proteins in acute cardiorenal syndrome
Description	Acute cardiorenal syndrome (CRS-1) is a morbid complication of acute cardiovascular disease. Mechanistic investigations have focused on intrarenal cellular signaling induced by ischemia/reperfusion. Additional cardiorenal connector signals have been postulated, but investigation in CRS-1 has been limited by a paucity of animal models and technical limitations precluding discovery studies of glomerular filtrate. To address these limitations, we developed a translational model of CRS-1, cardiac arrest and cardiopulmonary resuscitation (CA/CPR) in mice. Quantitative proteomics using isobaric tagging (10-plex TMT reagents, Thermo Fisher) was performed on 24h urine collections from mice with deficient tubular endocytosis and from littermate controls, before and after CA/CPR. Data acquisition used the SPS MS3 method on a Thermo Fusion Tribrid mass spectrometer for accurate reporter ion signals. The findings confirmed CA/CPR-specific cardiac proteins in urine and identified a novel CA/CPR-specific filtrate component: Cardiac LIM protein (CSRP3).
HostingRepository	PRIDE
AnnounceDate	2019-02-27
AnnouncementXML	Submission_2019-03-26_05:46:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-24 05:55:55	ID requested
1	2019-02-27 06:47:35	announced
⏵ 2	2019-03-26 05:46:39	announced	Updated publication reference for PubMed record(s): 30829647.

Wakasaki R, Matsushita K, Golgotiu K, Anderson S, Eiwaz MB, Orton DJ, Han SJ, Lee HT, Smith RD, Rodland KD, Piehowski PD, Hutchens MP, Glomerular filtrate proteins in acute cardiorenal syndrome. JCI Insight, 4(4):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Mouse model, cardiovascular disease, acute cardiorenal syndrome, urine, quantitative proteomics, isobaric labeling, tandem mass tags

Dr. Michael P. Hutchens
contact affiliation	Anesthesiology & Perioperative Medicine Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA
contact email	hutchenm@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012193

PRIDE project URI

• PRIDE
  1. PXD012193
     1. Label: PRIDE project
     2. Name: Glomerular filtrate proteins in acute cardiorenal syndrome