PXD012192

PXD012192 is an original dataset announced via ProteomeXchange.

Title	Peptidomics analysis of milk protein-derived peptides released over time in the preterm infant stomach
Description	Over the course of milk digestion, native milk proteases and infant digestive proteases fragment intact proteins into peptides with potential bioactivity. This study investigated the release of peptides over three hours of gastric digestion in 14 preterm infant sample sets. The peptide content was extracted and analyzed from milk and gastric samples via Orbitrap tandem mass spectrometry. The relative ion intensity (abundance) and count of peptides in each sample were compared over time and between infants fed milk fortified with bovine milk fortifier and infants fed unfortified milk. Bioactivity of the identified peptides was predicted by sequence homology to known bioactive milk peptides. Both total and bioactive peptide abundance and count continuously increased over three hours of gastric digestion. After accounting for infant weight, length, and post-conceptual age, fortification of milk limited the release of peptides from human milk proteins. Peptides that survived further gastric digestion after their initial release were structurally more similar to bioactive peptides than non-surviving peptides. This work is the first to provide a comprehensive profile of milk peptides released during gastric digestion over time, which is an essential step in determining which peptides are most likely to be biologically relevant in the infant.
HostingRepository	PRIDE
AnnounceDate	2019-03-12
AnnouncementXML	Submission_2019-03-11_21:56:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Beverly
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-24 05:53:58	ID requested
⏵ 1	2019-03-11 21:56:51	announced

Beverly RL, Underwood MA, Dallas DC, Peptidomics Analysis of Milk Protein-Derived Peptides Released over Time in the Preterm Infant Stomach. J Proteome Res, 18(3):912-922(2019) [pubmed]

curator keyword: Biological

submitter keyword: Peptide, Human milk, Gastric, Preterm, Bioactive, Digestion

David Dallas
contact affiliation	Nutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University
contact email	dave.dallas@oregonstate.edu
lab head
Robert Beverly
contact affiliation	Oregon State University
contact email	beverlyr@oregonstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012192
     1. Label: PRIDE project
     2. Name: Peptidomics analysis of milk protein-derived peptides released over time in the preterm infant stomach