PXD012186

PXD012186 is an original dataset announced via ProteomeXchange.

Title	Impact of HPV16 E6 and E7 oncogenes together with Chlamydia trachomatis infection on proteome changes in immortalized oral keratinocytes
Description	Certain types of Human papilloma viruses (HPV) are the etiological agents for cervical cancer. However, not all infections of high-risk HPVs will finally lead to cancer since most HPV infections are cleared without any consequences. Chlamydia trachomatis is the most prevalent sexual transmitted bacteria and is an obligatory intracellular pathogen exhibiting tropism in endocervical epithelial cells. Over the past decades, C. trachomatis is thought to be a potential co-factor for cervical cancer formation, but there are also studies that did not show such a correlation. To address this question in molecular terms, we stably expressed HPV16 E6 and E7 in spontaneously immortalized NOKs (normal oral keratinocytes) and performed SILAC (stable isotope labeling by amino acids in cell culture) with or without C. trachomatis infection to study the impact of HPV16 oncogene expression and C. trachomatis infection on host proteome changes.
HostingRepository	PRIDE
AnnounceDate	2019-03-18
AnnouncementXML	Submission_2019-04-02_04:12:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Klimentova
SpeciesList	scientific name: Chlamydia trachomatis; NCBI TaxID: 813; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human papillomavirus type 16; NCBI TaxID: 333760;
ModificationList	isotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-24 02:40:39	ID requested
1	2019-03-18 09:26:52	announced
⏵ 2	2019-04-02 04:12:40	announced	Updated publication reference for PubMed record(s): 30918060.

Yang R, Klimentov, á J, G, ö, ckel-Krzikalla E, Ly R, Gmelin N, Hotz-Wagenblatt A, Ř, ehulkov, á H, Stul, í, k J, R, ö, sl F, Niebler M, Combined Transcriptome and Proteome Analysis of Immortalized Human Keratinocytes Expressing Human Papillomavirus 16 (HPV16) Oncogenes Reveals Novel Key Factors and Networks in HPV-Induced Carcinogenesis. mSphere, 4(2):(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Human papillomavirus, Chlamydia trachomatis, cervical cancer

Martina Niebler
contact affiliation	Division of Viral Transformation Mechanisms, German Cancer Research Center (DKFZ), Heidelberg, Germany
contact email	m.niebler@dkfz-heidelberg.de
lab head
Jana Klimentova
contact affiliation	Faculty of Military Health Sciences, University of Defense, Hradec Kralove, Czech Republic
contact email	jana.klimentova@unob.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012186

PRIDE project URI

• PRIDE
  1. PXD012186
     1. Label: PRIDE project
     2. Name: Impact of HPV16 E6 and E7 oncogenes together with Chlamydia trachomatis infection on proteome changes in immortalized oral keratinocytes