PXD012175

PXD012175 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of Cx43 expressing cells
Description	Extracellular matrix remodeling, degradation and glioma cell motility are critical aspects of glioblastoma multiforme (GBM). Despite being a rich source of potential biomarkers and targets for therapeutic advance, the dynamic changes within the extracellular environment that are specific to GBM cell motility have yet to be fully resolved. The gap junction protein connexin43 (Cx43) increases glioma migration and invasion in a variety of in vitro and in vivo models. In this study, the conditioned media of Cx43-expressing C6 rat glioma cells was found to increase the motility of the parental line. Demonstrating the selective engagement of ECM-remodelling pathways, secretome analysis revealed the near-binary expression of osteopontin and matrix metalloproteinase-3 (MMP3).
HostingRepository	PRIDE
AnnounceDate	2019-04-17
AnnouncementXML	Submission_2019-04-17_05:35:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vincent Chen
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	6530B Q-TOF LC/MS; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-24 02:10:15	ID requested
⏵ 1	2019-04-17 05:35:36	announced

Aftab Q, Mesnil M, Ojefua E, Poole A, Noordenbos J, Strale PO, Sitko C, Le C, Stoynov N, Foster LJ, Sin WC, Naus CC, Chen VC, Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for Glioma Migration and MMP3 Activation. Front Neurosci, 13():143(2019) [pubmed]

curator keyword: Biological

submitter keyword: Glioma, secretome, connexin, connexin43

Vincent Chen
contact affiliation	chenlab.ca Brandon University
contact email	chenv@brandonu.ca
lab head
Vincent Chen
contact affiliation	Brandon University
contact email	chenv@Brandonu.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012175

PRIDE project URI

• PRIDE
  1. PXD012175
     1. Label: PRIDE project
     2. Name: Secretome analysis of Cx43 expressing cells