PXD012163

PXD012163 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of CENP-A on serine 7 does not control centromere function
Description	CENP-A is the histone H3 variant necessary to specify the location of all eukaryoticcentromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7,has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function.
HostingRepository	PRIDE
AnnounceDate	2019-01-28
AnnouncementXML	Submission_2019-01-28_07:53:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ANDREA SCELFO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-21 08:52:06	ID requested
1	2019-01-28 05:06:50	announced
⏵ 2	2019-01-28 07:53:12	announced	Updated publication reference for PubMed record(s): 30635586.

Barra V, Logsdon GA, Scelfo A, Hoffmann S, Herv, é S, Aslanian A, Nechemia-Arbely Y, Cleveland DW, Black BE, Fachinetti D, Phosphorylation of CENP-A on serine 7 does not control centromere function. Nat Commun, 10(1):175(2019) [pubmed]

curator keyword: Biological

submitter keyword: centromere, Cenp-A, phosphorilation, cell cycle

Daniele Fachinetti
contact affiliation	Institut Curie, PSL Research University, CNRS, UMR 144, 26 rue d’Ulm, F-75005, Paris, France.
contact email	daniele.fachinetti@curie.fr
lab head
ANDREA SCELFO
contact affiliation	INSTITUT CURIE
contact email	andrea.scelfo@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD012163

PRIDE project URI

• PRIDE
  1. PXD012163
     1. Label: PRIDE project
     2. Name: Phosphorylation of CENP-A on serine 7 does not control centromere function