PXD012152

PXD012152 is an original dataset announced via ProteomeXchange.

Title	Proteomic and genomic analysis of the marine fungus Paradendryphiella salina
Description	We set out to investigate the genetic adaptions of the known marine fungus Paradendryphiella salina CBS112865 to the degradation of brown macro-algae, expecting to find a repertoire of carbohydrate active enzymes highly specialized to the degradation of algal polysaccharides. We performed whole genome, transcriptome sequencing and shotgun proteomic analysis of the secretome of P. salina growing on three species of brown algae and under carbon starvation. The genome comparison to close terrestrial fungal relatives, revealed P. salina to have a similar, but reduced carbohydrate active enzyme (CAZyme) profile, except for the presence of three putative alginate lyase 7 genes, most likely acquired via ancient horizontal gene transfer event from a marine bacterium and a polysaccharide lyase 8 gene with similarity to ascomycete chondroitin AC lyases. The proteomic analysis revealed both PL7 and PL8 enzymes to be highly abundant in the algal fermentations together with enzymes necessary for degradation of laminarin, cellulose, lipids and peptides. Our findings indicate that the base CAZyme repertoire of saprobic and plant pathogenic ascomycetes with the necessary addition of alginate lyases provide the fungi with the enzymatic capabilities to thrive on brown algae polysaccharides and even cope with the algal defense mechanisms.
HostingRepository	PRIDE
AnnounceDate	2019-11-13
AnnouncementXML	Submission_2019-11-12_16:28:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Pilgaard
SpeciesList	scientific name: Paradendryphiella salina; NCBI TaxID: 179392;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-21 04:11:11	ID requested
⏵ 1	2019-11-12 16:28:26	announced

Pilgaard B, Wilkens C, Herbst FA, Vuillemin M, Rhein-Knudsen N, Meyer AS, Lange L, Proteomic enzyme analysis of the marine fungus Paradendryphiella salina reveals alginate lyase as a minimal adaptation strategy for brown algae degradation. Sci Rep, 9(1):12338(2019) [pubmed]

curator keyword: Biological

submitter keyword: marine, macro-algae, fungi, LC-MS/MS, supernatant

Anne S. Meyer
contact affiliation	Bioengineering, Protein Chemistry & Enzyme Technology, Technical Univesity of Denmark (DTU), Denmark
contact email	asme@dtu.dk
lab head
Bo Pilgaard
contact affiliation	Technical university of Denmark
contact email	bpil@dtu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012152
     1. Label: PRIDE project
     2. Name: Proteomic and genomic analysis of the marine fungus Paradendryphiella salina