PXD012151

PXD012151 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of focal cerebral ischemia/reperfusion injury in rats
Description	Stroke is a leading cause of mortality and long-term disability and ischemic stroke accounts for 87% of all strokes. Though timely recanalization of the occluded vessel is essential in the treatment of ischemic stroke, it is well known to cause ischemia-reperfusion (I/R) injury which result in neuronal cell death, brain tissue loss and severe neurological deficits. In this work, we employed a global proteomic approach to examine the changes of cerebral cortex proteins in rats undergoing acute and long-term I/R injury. In vivo middle cerebral artery occlusion (MCAO) model of focal cerebral I/R injury in rats was established. The animals were divided into three model groups with 2 h-MCAO followed with different reperfusion time, 1 day, 7 days and 14 days, respectively. For each model group a sham group was correspondingly set. Each group included four animals. For proteomic analysis, cerebral cortex proteins were extracted and analyzed by SDS-PAGE, whole-lane slicing, in-gel digestion and label-free quantitative LC-MS/MS. A total of 5621 proteins were identified and their quantities between the surgery and corresponding sham groups and across the three reperfusion time points were compared for mechanism investigation. This dataset includes all the raw files of the 840 LC-MS runs (6 groups x 4 animals x 35 gel squares/sample), as well as their identification and quantitation results at the levels of peptide fragments, peptides and proteins, respectively.
HostingRepository	PRIDE
AnnounceDate	2019-07-04
AnnouncementXML	Submission_2019-07-04_05:06:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Jin
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Bruker Daltonics maXis series

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-21 03:16:41	ID requested
⏵ 1	2019-07-04 05:07:28	announced

Wen M, Jin Y, Zhang H, Sun X, Kuai Y, Tan W, Proteomic Analysis of Rat Cerebral Cortex in the Subacute to Long-Term Phases of Focal Cerebral Ischemia-Reperfusion Injury. J Proteome Res, 18(8):3099-3118(2019) [pubmed]

submitter keyword: rat, brain stroke, middle cerebral artery occlusion (MCAO), ischemia-reperfusion injury, time course, proteomics

Ya Jin
contact affiliation	Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology
contact email	ya.jin@ymail.com
lab head
Ya Jin
contact affiliation	Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology
contact email	ya.jin@ymail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD012151

PRIDE project URI

• PRIDE
  1. PXD012151
     1. Label: PRIDE project
     2. Name: Proteomic analysis of focal cerebral ischemia/reperfusion injury in rats