PXD012136

PXD012136 is an original dataset announced via ProteomeXchange.

Title	Hepatocytes induces properties of wound healing type and regulatory macrophages in co-cultivated BMDM
Description	The liver comprises the body´s largest pool of macrophages constituting approximately 80 % of all sessile tissue macrophages of the body. After liver damage monocyte-derived macrophages are recruited into the liver and were here affected by a micro milieu which is considerably influenced by hepatocytes. Up to now the complex network that determines the differentiation and function of liver macrophages and the impact of the intercellular communication between macrophages and the other parenchymal and non-parenchymal cell types of the liver is not well understood. The present study investigates the role of the intercellular communication between macrophages and hepatocytes on macrophage differentiation and function. Under physiological conditions the sinusoidal endothelial cell layer and the space of Dissé separate macrophages and hepatocytes from each other. Therefore the study focuses on the impact of the intercellular communication via soluble mediators on the differentiation of bone marrow derived macrophages (BMDM), which were recruited to the liver after liver injury, in co-culture models with highly purified primary murine hepatocytes embedded in a sandwich collagen matrix.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:03:26.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-20 04:10:13	ID requested
1	2023-07-07 00:50:22	announced
⏵ 2	2023-11-14 07:03:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Macrophages, cell-cell communication,Liver, Hepatocytes, label-free quantification

Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory (MPL) Biologisch-Medizinisches Forschungszentrum (BMFZ) University hospital Düsseldorf Heinrich-Heine-Universität Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD012136

PRIDE project URI

• PRIDE
  1. PXD012136
     1. Label: PRIDE project
     2. Name: Hepatocytes induces properties of wound healing type and regulatory macrophages in co-cultivated BMDM