PXD012133

PXD012133 is an original dataset announced via ProteomeXchange.

Title	Evaluating cPILOT Performance on Orbitrap Instruments
Description	A global cPILOT assay developed on an Orbitrap Velos instrument was transitioned to an Orbitrap Fusion Lumos instrument. Parameters such as the LC gradient, m/z isolation window, dynamic exclusion, targeted mass analyses, and SPS-N were optimized. The number of proteins identified on the Fusion Lumos (1848) without fractionation are close to those identified to the Orbitrap Velos (2199) with sample pre-fractionation. Additionally, including sample fractionation with the Fusion Lumos optimized parameters resulted in the identification of 4968 proteins, of which 1234 were quantified in each tissue type and genotype (N=5 or 6). Combined, the various experiments described herein informed of system-wide changes in brain, heart, and liver proteins from a late-stage amyloid precursor protein/presenilin-1 (APP/PS-1) human transgenic double knock – in mouse model of AD.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_09:52:53.614.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina King
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-20 00:23:31	ID requested
⏵ 1	2021-09-08 09:52:54	announced

King CD, Robinson RAS, Evaluating Combined Precursor Isotopic Labeling and Isobaric Tagging Performance on Orbitraps To Study the Peripheral Proteome of Alzheimer's Disease. Anal Chem, 92(4):2911-2916(2020) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: cPILOT, proteomics, multiplexing, Fusion Lumos, Alzheimer's disease

Rena A.S. Robinson
contact affiliation	Associate Professor, Department of Chemistry, Vanderbilt University
contact email	rena.as.robinson@vanderbilt.edu
lab head
Christina King
contact affiliation	University of Pittsburgh
contact email	cdk33@pitt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD012133

PRIDE project URI

• PRIDE
  1. PXD012133
     1. Label: PRIDE project
     2. Name: Evaluating cPILOT Performance on Orbitrap Instruments