PXD012127

PXD012127 is an original dataset announced via ProteomeXchange.

Title	TEAD1-DBD-DNA interaction studied by structural MS
Description	TEAD transcription factors mediate gene expression regulation through interactions with their DNA response M-CAT motif. They are active mainly during growth and development and induce gene expression of proteins involved in cell proliferation, differentiation or apoptosis prevention. TEADs and many of their target proteins are known to be upregulated in several types of cancers. To study the interaction of DNA binding domain of TEAD1 with M-CAT motifs of different orientations originating from regulatory regions of human genes (CTGF, SRF, C-MYC and GLUT1), dissociation constant of each complex was determined using fluorescence anisotropy assay. Structural mass spectrometry techniques (H/D exchange and quantitative chemical cross-linking) and molecular docking were utilized for structural characterization of the binding interface. In-vitro results were correlated with situation in living cells studied by ChIP and Q-PCR.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-13_16:48:46.520.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruzena Filandrova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics solarix series

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-20 00:05:04	ID requested
1	2020-12-07 22:32:39	announced
⏵ 2	2022-02-13 16:48:46	announced	2022-02-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: EPIC-XS

submitter keyword: TEAD1, DNA, Mass spectrometry, M-CAT, H/D exchange,Transcription factor, Chemical cross-linking

Petr Novak
contact affiliation	Laboratory of Structural Biology and Cell Signaling, Institute of Microbiology, The Czech Academy of Sciences, Prague, 14220, Czech Republic
contact email	pnovak@biomed.cas.cz
lab head
Ruzena Filandrova
contact affiliation	Institute of Microbiology of the CAS
contact email	ruzena.liskova@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD012127

PRIDE project URI

• PRIDE
  1. PXD012127
     1. Label: PRIDE project
     2. Name: TEAD1-DBD-DNA interaction studied by structural MS