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PXD012117

PXD012117 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeuterium-free, Three-plexed Peptide Di-Ethylation for Highly Accurate Quantitative Proteomics
DescriptionThe deuterium, frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. Herein, we introduce a novel three-plexed peptide ‘di-ethylation’ using only 13C-isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the conventional deuterium-based di-methylation labeling in both a single shot and multidimensional LC-MS/MS analysis of HeLa proteome. Furthermore, in time-resolved profiling of Xenopus laevis early embryogenesis, our 3-plexed di-ethylation outperformed isobaric labeling approaches in terms of quantification accuracy or number of protein identification, generating >2 times more differentially expressed proteins. Our cost-effective and highly accurate 3-plexed di-ethylation method could contribute to various types of quantitative proteomics applications, in which three of multiplexity would be sufficient.
HostingRepositoryPRIDE
AnnounceDate2019-01-17
AnnouncementXMLSubmission_2019-01-17_08:45:08.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJae Hun Jung
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-19 02:49:51ID requested
12019-01-17 08:45:09announced
Publication List
Jung J, Jeong K, Choi Y, Kim SA, Kim H, Lee JW, Kim VN, Kim KP, Kim JS, Deuterium-Free, Three-Plexed Peptide Diethylation for Highly Accurate Quantitative Proteomics. J Proteome Res, 18(3):1078-1087(2019) [pubmed]
Keyword List
submitter keyword: di-ethylation, di-methylation, quantitative accuracy, deuterium effect, isotopic labeling, isobaric labeling
Contact List
Jaehun Jung
contact affiliationKyung Hee University
contact emailultramanyo@naver.com
lab head
Jae Hun Jung
contact affiliationKHU
contact emailultramanyo@naver.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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