PXD012117 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deuterium-free, Three-plexed Peptide Di-Ethylation for Highly Accurate Quantitative Proteomics |
Description | The deuterium, frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. Herein, we introduce a novel three-plexed peptide ‘di-ethylation’ using only 13C-isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the conventional deuterium-based di-methylation labeling in both a single shot and multidimensional LC-MS/MS analysis of HeLa proteome. Furthermore, in time-resolved profiling of Xenopus laevis early embryogenesis, our 3-plexed di-ethylation outperformed isobaric labeling approaches in terms of quantification accuracy or number of protein identification, generating >2 times more differentially expressed proteins. Our cost-effective and highly accurate 3-plexed di-ethylation method could contribute to various types of quantitative proteomics applications, in which three of multiplexity would be sufficient. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-17 |
AnnouncementXML | Submission_2019-01-17_08:45:08.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jae Hun Jung |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-19 02:49:51 | ID requested | |
⏵ 1 | 2019-01-17 08:45:09 | announced | |
Publication List
Jung J, Jeong K, Choi Y, Kim SA, Kim H, Lee JW, Kim VN, Kim KP, Kim JS, Deuterium-Free, Three-Plexed Peptide Diethylation for Highly Accurate Quantitative Proteomics. J Proteome Res, 18(3):1078-1087(2019) [pubmed] |
Keyword List
submitter keyword: di-ethylation, di-methylation, quantitative accuracy, deuterium effect, isotopic labeling, isobaric labeling |
Contact List
Jaehun Jung |
contact affiliation | Kyung Hee University |
contact email | ultramanyo@naver.com |
lab head | |
Jae Hun Jung |
contact affiliation | KHU |
contact email | ultramanyo@naver.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012117
- Label: PRIDE project
- Name: Deuterium-free, Three-plexed Peptide Di-Ethylation for Highly Accurate Quantitative Proteomics