PXD012113

PXD012113 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteome and proteome analysis emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis
Description	Phosphorylation is an important event in cell signaling that is modulated by kinases and phosphatases. In T. cruzi, the etiological agent of Chagas disease, approximately 2% of the genome comprises protein kinases. This parasite has a heteroxenic life cycle with four different development stages. In the midgut of triatomine, epimastigotes differentiate into metacyclic trypomastigotes in a process known as metacyclogenesis. This process can be reproduced in vitro by submitting parasites to nutritional stress (NS). Aiming to contribute to the elucidation of mechanisms that trigger metacyclogenesis, we applied super-SILAC (super-stable isotope labeling by amino acids in cell culture) and LC-MS/MS to analyze different points during NS. This analysis resulted in the identification of 4,205 protein groups and 3,643 phosphopeptides with the location of 4,846 phosphorylation sites. Several phosphosites were considered modulated along NS and are present in proteins associated with various functions, such as fatty acid synthesis and the regulation of protein expression, reinforcing the importance of phosphorylation and signaling events to the parasite. These modulated sites may be triggers of metacyclogenesis.
HostingRepository	PRIDE
AnnounceDate	2019-08-05
AnnouncementXML	Submission_2019-08-05_02:35:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aline Lucena
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap XL ETD; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-19 02:46:07	ID requested
⏵ 1	2019-08-05 02:35:56	announced

Lucena ACR, Amorim JC, de Paula Lima CV, Batista M, Krieger MA, de Godoy LMF, Marchini FK, Quantitative phosphoproteome and proteome analyses emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis. Cell Stress Chaperones, 24(5):927-936(2019) [pubmed]

submitter keyword: Trypanosoma cruzi, metacyclogenesis, nutritional stress, proteomics, phosphoproteomics, super-SILAC

Fabricio Klerynton Marchini
contact affiliation	Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil
contact email	fabricio.marchini@fiocruz.br
lab head
Aline Lucena
contact affiliation	Carlos Chagas Institute
contact email	aline_cr2102@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012113
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteome and proteome analysis emphasize the influence of phosphorylation events during the nutritional stress of Trypanosoma cruzi: the initial moments of in vitro metacyclogenesis