PXD012100

PXD012100 is an original dataset announced via ProteomeXchange.

Title	Cyclin A triggers Mitosis either via Greatwall or Cyclin B
Description	Two mitotic Cyclins, A and B, exist in higher eukaryotes, but their specialised functions in mitosis are poorly understood. Using degron tags we analyse how acute depletion of these proteins affects mitosis. Loss of Cyclin A in G2-phase prevents the initial activation of Cdk1. Cells lacking Cyclin B can enter mitosis and phosphorylate most mitotic proteins, because of parallel PP2A:B55 phosphatase inactivation by Greatwall kinase. The final barrier to mitotic establishment corresponds to nuclear envelope breakdown that requires a decisive shift in the balance of Cdk1 and PP2A:B55 activity. Beyond this point Cyclin B/Cdk1 is essential to phosphorylate a distinct subset mitotic Cdk1 substrates that are essential to complete cell division. Our results identify how Cyclin A, B and Greatwall coordinate mitotic progression by increasing levels of Cdk1-dependent substrate phosphorylation. The phosphoproteomics dataset aims to identify substrates that are affected specifically by acute depletion of Cyclin Bprotein.
HostingRepository	PRIDE
AnnounceDate	2020-05-21
AnnouncementXML	Submission_2020-05-21_00:54:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tony Ly
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-18 08:53:00	ID requested
⏵ 1	2020-05-21 00:54:38	announced

Dataset with its publication pending

submitter keyword: Human, Cell Cycle, Phosphoproteomics, Cyclin, Mitosis

Tony Ly
contact affiliation	Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh
contact email	tly@ed.ac.uk
lab head
Tony Ly
contact affiliation	University of Edinburgh
contact email	tly@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012100
     1. Label: PRIDE project
     2. Name: Cyclin A triggers Mitosis either via Greatwall or Cyclin B