PXD012096

PXD012096 is an original dataset announced via ProteomeXchange.

Title	RUNX2 stimulates neoangiogenesis through the RUNT domain in melanoma
Description	Transcription factors, by acting on gene expression can affect molecular pathways and can thereby induce malignant transformation. Among the transcription factors involved in cellular transformation, we recently highlighted the role of RUNX2 in melanoma cell migration and proliferation. In the present study, we extended the analysis of the molecular effects of RUNT domain of RUNX2 in melanoma cells to deepen understanding of the underlying mechanisms. By the CRISPR/Cas9 system we generated the RUNT KO melanoma cells 3G8. Therefore, using a proteomic approach, we compared 3G8 with the original wild-type melanoma cell line (A375). The results showed a specific protein signature of 3G8 cells related to apoptosis and migration. In addition, proteomic analysis allowed us to point out the involvement of the RUNT domain in the neoangiogenesis process. Among the deregulated proteins implicated in angiogenesis we found fatty acid synthase, chloride intracellular channel protein-4, heat shock protein beta-1, Rho guanine nucleotide exchange factor 1, D-3-phosphoglycerate dehydrogenase, myosin-1c and caveolin-1. Accordingly, the VEGF gene expression was higher in A375 compared to 3G8 cells; HUVEC cells expressed increased levels of the neoangiogenetic markers CD105 and CD31 when co-cultured with A375 cells. In conclusion, our study provided new insight into RUNX2 molecular details which can be crucial to possibly propose it as an oncotarget of melanoma.
HostingRepository	PRIDE
AnnounceDate	2019-11-13
AnnouncementXML	Submission_2019-11-12_16:22:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcello Manfredi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-18 08:36:34	ID requested
⏵ 1	2019-11-12 16:22:02	announced

Cecconi D, Brandi J, Manfredi M, Serena M, Dalle Carbonare L, Deiana M, Cheri S, Parolini F, Gandini A, Marchetto G, Innamorati G, Avanzi F, Antoniazzi F, Marengo E, Tiso N, Mottes M, Zipeto D, Valenti MT, Runx2 stimulates neoangiogenesis through the Runt domain in melanoma. Sci Rep, 9(1):8052(2019) [pubmed]

submitter keyword: Melanoma, RUNX2, Proteomics, Neoangiogenesis

Marcello Manfredi
contact affiliation	University of Eastern Piedmont Department of Sciences and Technological Innovation, University of Piemonte Orientale, Italy
contact email	marcello.manfredi@uniupo.it
lab head
Marcello Manfredi
contact affiliation	University of Eastern Piedmont
contact email	marcello.manfredi@uniupo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD012096

PRIDE project URI

• PRIDE
  1. PXD012096
     1. Label: PRIDE project
     2. Name: RUNX2 stimulates neoangiogenesis through the RUNT domain in melanoma