PXD012089

PXD012089 is an original dataset announced via ProteomeXchange.

Title	Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism
Description	Mitochondrial dysfunction is one of many key factors in the etiology of alcoholic liver disease (ALD). Lysine acetylation is known to regulate numerous mitochondrial metabolic pathways and recent reports demonstrate that alcohol-induced protein acylation negatively impacts these processes. To identify regulatory mechanisms attributed to alcohol-induced protein post-translational modifications, we employed a model of alcohol consumption within the context of wild type (WT), sirtuin 3 knockout (SIRT3 KO), and sirtuin 5 knockout(SIRT5 KO) mice to manipulate hepatic mitochondrial protein acylation. Mitochondrial fractions were examined by label-free quantitative HPLC-MS/MS to reveal competition between lysine acetylation and succinylation. A class of proteins defined as “differential acyl switching proteins” demonstrate select sensitivity to alcohol-induced protein acylation. A number of these proteins reveal saturated lysine-site occupancy, suggesting a significant level of differential stoichiometry in the setting of ethanol consumption. We hypothesize that ethanol downregulates numerous mitochondrial metabolic pathways through differential acyl switching proteins.
HostingRepository	PRIDE
AnnounceDate	2019-01-23
AnnouncementXML	Submission_2019-01-23_00:42:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cole Michel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; deaminated residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-17 07:15:43	ID requested
⏵ 1	2019-01-23 00:42:16	announced

Ali HR, Assiri MA, Harris PS, Michel CR, Yun Y, Marentette JO, Huynh FK, Orlicky DJ, Shearn CT, Saba LM, Reisdorph R, Reisdorph N, Hirschey MD, Fritz KS, Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism. J Proteome Res, 18(4):1513-1531(2019) [pubmed]

submitter keyword: Acylation, Acetylation, Succinylation, Mitochondira, Ethanol Metabolism

Kristofer Scott Fritz
contact affiliation	Department of Pharmaceutical Sciences Skaggs School of Pharmacy and Pharmaceutical Sciences University of Colorado Anschutz Medical Campus
contact email	kristofer.fritz@ucdenver.edu
lab head
Cole Michel
contact affiliation	University of Colorado Skaggs School of Pharmacy
contact email	cole.michel@ucdenver.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD012089

PRIDE project URI

• PRIDE
  1. PXD012089
     1. Label: PRIDE project
     2. Name: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism