PXD012089 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism |
Description | Mitochondrial dysfunction is one of many key factors in the etiology of alcoholic liver disease (ALD). Lysine acetylation is known to regulate numerous mitochondrial metabolic pathways and recent reports demonstrate that alcohol-induced protein acylation negatively impacts these processes. To identify regulatory mechanisms attributed to alcohol-induced protein post-translational modifications, we employed a model of alcohol consumption within the context of wild type (WT), sirtuin 3 knockout (SIRT3 KO), and sirtuin 5 knockout(SIRT5 KO) mice to manipulate hepatic mitochondrial protein acylation. Mitochondrial fractions were examined by label-free quantitative HPLC-MS/MS to reveal competition between lysine acetylation and succinylation. A class of proteins defined as “differential acyl switching proteins” demonstrate select sensitivity to alcohol-induced protein acylation. A number of these proteins reveal saturated lysine-site occupancy, suggesting a significant level of differential stoichiometry in the setting of ethanol consumption. We hypothesize that ethanol downregulates numerous mitochondrial metabolic pathways through differential acyl switching proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-23 |
AnnouncementXML | Submission_2019-01-23_00:42:15.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cole Michel |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | carbamoylated residue; acetylated residue; monohydroxylated residue; deaminated residue |
Instrument | 6520 Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-17 07:15:43 | ID requested | |
⏵ 1 | 2019-01-23 00:42:16 | announced | |
Publication List
Ali HR, Assiri MA, Harris PS, Michel CR, Yun Y, Marentette JO, Huynh FK, Orlicky DJ, Shearn CT, Saba LM, Reisdorph R, Reisdorph N, Hirschey MD, Fritz KS, Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism. J Proteome Res, 18(4):1513-1531(2019) [pubmed] |
Keyword List
submitter keyword: Acylation, Acetylation, Succinylation, Mitochondira, Ethanol Metabolism |
Contact List
Kristofer Scott Fritz |
contact affiliation | Department of Pharmaceutical Sciences Skaggs School of Pharmacy and Pharmaceutical Sciences University of Colorado Anschutz Medical Campus |
contact email | kristofer.fritz@ucdenver.edu |
lab head | |
Cole Michel |
contact affiliation | University of Colorado Skaggs School of Pharmacy |
contact email | cole.michel@ucdenver.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012089
- Label: PRIDE project
- Name: Quantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism