⮝ Full datasets listing

PXD012089

PXD012089 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantifying Competition Among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism
DescriptionMitochondrial dysfunction is one of many key factors in the etiology of alcoholic liver disease (ALD). Lysine acetylation is known to regulate numerous mitochondrial metabolic pathways and recent reports demonstrate that alcohol-induced protein acylation negatively impacts these processes. To identify regulatory mechanisms attributed to alcohol-induced protein post-translational modifications, we employed a model of alcohol consumption within the context of wild type (WT), sirtuin 3 knockout (SIRT3 KO), and sirtuin 5 knockout(SIRT5 KO) mice to manipulate hepatic mitochondrial protein acylation. Mitochondrial fractions were examined by label-free quantitative HPLC-MS/MS to reveal competition between lysine acetylation and succinylation. A class of proteins defined as “differential acyl switching proteins” demonstrate select sensitivity to alcohol-induced protein acylation. A number of these proteins reveal saturated lysine-site occupancy, suggesting a significant level of differential stoichiometry in the setting of ethanol consumption. We hypothesize that ethanol downregulates numerous mitochondrial metabolic pathways through differential acyl switching proteins.
HostingRepositoryPRIDE
AnnounceDate2019-01-23
AnnouncementXMLSubmission_2019-01-23_00:42:15.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCole Michel
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListcarbamoylated residue; acetylated residue; monohydroxylated residue; deaminated residue
Instrument6520 Quadrupole Time-of-Flight LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-17 07:15:43ID requested
12019-01-23 00:42:16announced
Publication List
Ali HR, Assiri MA, Harris PS, Michel CR, Yun Y, Marentette JO, Huynh FK, Orlicky DJ, Shearn CT, Saba LM, Reisdorph R, Reisdorph N, Hirschey MD, Fritz KS, Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism. J Proteome Res, 18(4):1513-1531(2019) [pubmed]
Keyword List
submitter keyword: Acylation, Acetylation, Succinylation, Mitochondira, Ethanol Metabolism
Contact List
Kristofer Scott Fritz
contact affiliationDepartment of Pharmaceutical Sciences Skaggs School of Pharmacy and Pharmaceutical Sciences University of Colorado Anschutz Medical Campus
contact emailkristofer.fritz@ucdenver.edu
lab head
Cole Michel
contact affiliationUniversity of Colorado Skaggs School of Pharmacy
contact emailcole.michel@ucdenver.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD012089
PRIDE project URI
Repository Record List
[ + ]