PXD012084

PXD012084 is an original dataset announced via ProteomeXchange.

Title	Quantifying Positional Isomers (QPI) by top-down mass spectrometry
Description	Proteomics has exposed a plethora of post-translational modifications, but demonstrating functional relevance requires new approaches. Top-down proteomics can characterize co-occurring modifications in terms of localization, abundance and hierarchy. Here, we present a top-down MS analysis workflow for the discovery and quantification of proteoforms. Confident fragment assignment allows for localization of modification sites and quantification of all proteoforms, including positional isomers, as validated by investigating synthetic isoforms of ubiquitin and hyper-phosphorylated Bora.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:16:04.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RichardScheltema
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-17 07:12:55	ID requested
1	2021-03-18 01:54:13	announced
⏵ 2	2023-05-14 14:16:05	announced	2023-05-14: Updated project metadata.

Brunner AM, L, ö, ssl P, Geurink PP, Ovaa H, Albanese P, Altelaar AFM, Heck AJR, Scheltema RA, Quantifying Positional Isomers (QPI) by Top-Down Mass Spectrometry. Mol Cell Proteomics, 20():100070(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: Top-down mass spectrometry, positional isomers, quantitation, phosphorylation

RichardScheltema
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences Padualaan 8 3584 CH Utrecht Utrecht University The Netherlands
contact email	r.a.scheltema@uu.nl
lab head
RichardScheltema
contact affiliation	Utrecht University
contact email	r.a.scheltema@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD012084

PRIDE project URI

• PRIDE
  1. PXD012084
     1. Label: PRIDE project
     2. Name: Quantifying Positional Isomers (QPI) by top-down mass spectrometry