PXD012080

PXD012080 is an original dataset announced via ProteomeXchange.

Title	Freshly isolated rat liver sinusoidal endothelial cells and Kupffer cells, LC-MSMS
Description	Liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs) have important roles in liver homeostasis and host defense. Sharing the same microenvironment in the liver sinusoid, they form an effective scavenger cell system for removal of potentially harmful blood-borne substances. Unlike most other endothelia, LSECs are highly efficient in endocytosis of nanoparticles, including virus. Though controversial, LSECs have been reported to act as antigen presenting cells, thus contributing importantly to induction of immune tolerance in liver. There are also controversies about LSEC and KC specific markers, which may be due to overlapping cell functions, species differences, and/or problems with cell purification. We therefore used label-free proteomics to characterize and quantitatively compare proteome of freshly isolated, highly pure rat LSECs (SE-1/CD32b positive) and KCs (CD11b/c positive.We found that most immune genes expressed in KCs were also expressed in LSECs, albeit at a lower density, and they also have overlap in cell surface marker expression. Both cell types express high levels of scavenger receptors and immune lectins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:14:52.065.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabin Bhandari
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-17 06:24:05	ID requested
1	2020-11-11 07:09:43	announced
⏵ 2	2024-10-22 05:14:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: liver cells, macrophage,rat, immunity, endothelium

Karen Kristine Sørensen
contact affiliation	Institute of Medical Biology, Faculty of Health, UiT/The Arctic University of Norway
contact email	karen.sorensen@uit.no
lab head
Sabin Bhandari
contact affiliation	UiT
contact email	sabin.bhandari@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD012080

PRIDE project URI

• PRIDE
  1. PXD012080
     1. Label: PRIDE project
     2. Name: Freshly isolated rat liver sinusoidal endothelial cells and Kupffer cells, LC-MSMS