PXD012078

PXD012078 is an original dataset announced via ProteomeXchange.

Title	Substrates of human Sec62/Sec63 complex
Description	The Sec61-associated Sec62/Sec63 complex supports translocation of only a subset of precursor polypeptides, i.e. in a substrate-specific manner. To characterize Sec62/Sec63-dependent precursors, we combined siRNA-mediated Sec62 or Sec63 depletion in Hela cells or CRISPR/Cas9-mediated knock-down in HEK 293 cells with label-free quantitative proteomics and differential expression analysis.
HostingRepository	PRIDE
AnnounceDate	2020-03-06
AnnouncementXML	Submission_2020-03-06_04:14:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Zimmermann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-17 04:01:39	ID requested
⏵ 1	2020-03-06 04:14:58	announced

Schorr S, Nguyen D, Ha, ß, denteufel S, Nagaraj N, Cavali, é A, Greiner M, Weissgerber P, Loi M, Paton AW, Paton JC, Molinari M, F, ö, rster F, Dudek J, Lang S, Helms V, Zimmermann R, Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import. FEBS J, 287(21):4612-4640(2020) [pubmed]

curator keyword: Biological

submitter keyword: HeLa, HEK 293, ER protein import

Prof. Dr. Richard Zimmermann (lab head)
contact affiliation	Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, Kirrbergerstr. 100, 66421 Homburg, Deutschland, fone +49 (0)6841 1626510, fax +49 (0)6841 1626288 Nagarjuna Nagaraj, Max Planck Institute for Biochemistry, Core facility (contact)
contact email	richard.zimmermann@uks.eu
lab head
Richard Zimmermann
contact affiliation	Saarland University
contact email	richard.zimmermann@uks.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD012078

PRIDE project URI

• PRIDE
  1. PXD012078
     1. Label: PRIDE project
     2. Name: Substrates of human Sec62/Sec63 complex