PXD012061

PXD012061 is an original dataset announced via ProteomeXchange.

Title	Comparison of the composition and interaction partners of the Nucleosome Remodeling and Deacetylase (NuRD) complex in cancer cells
Description	The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for normal development in metazoans and has direct implications in a range of human diseases. Amongst all classes of chromatin remodeling complexes, the NuRD complex has been the most recalcitrant to analysis. This is, in part, due to difficulties with producing the intact complex recombinantly. Using affinity-purification followed by mass spectrometry, we determined the stoichiometry of the NuRD complex from three cancer cell lines representing three different human tissues. We also analysed the data to determine the interactome of the NuRD complex and identified potential NuRD interactors.
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-03-13_14:26:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Low
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-14 01:54:08	ID requested
⏵ 1	2019-03-13 14:26:55	announced

Sharifi Tabar M, Mackay JP, Low JKK, The stoichiometry and interactome of the Nucleosome Remodeling and Deacetylase (NuRD) complex are conserved across multiple cell lines. FEBS J, 286(11):2043-2061(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: NuRD, stoichiometry, iBAQ, interactome, shotgun proteomics

Joel Mackay
contact affiliation	School of Life and Environmental Sciences, University of Sydney, Australia
contact email	joel.mackay@sydney.edu.au
lab head
Jason Low
contact affiliation	The University of Sydney
contact email	jason.low@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012061
     1. Label: PRIDE project
     2. Name: Comparison of the composition and interaction partners of the Nucleosome Remodeling and Deacetylase (NuRD) complex in cancer cells