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PXD012058 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDefining the T cell proteome and the role of mTORC1 during differentiation using quantitative proteomics
DescriptionHigh-resolution quantitative mass-spectrometry reveals similarities and disparities in how murine naïve CD4 and CD8 T cells respond to immune activation and re-structure proteomes as they differentiate to effector cells. The data map core transcriptional, metabolic and protein synthesis machinery, nutrient transporters and environment sensing molecules and reveal differences in the biosynthetic capacity of CD4 and CD8 T cells. One key nutrient sensing kinase is mammalian target of rapamycin complex1 (mTORC1). The current study identifies common and distinct mTORC1 regulated processes and divergent outcomes of mTORC1 inhibition in naïve versus effector CD4 and CD8 T cell populations. The data provide a resource that maps how immune activation and mTORC1 reshape CD4 and CD8 T cell proteomes and highlights that a deep understanding of T cell phenotype requires modelling of the impact of immune regulators on protein copy number, cellular protein concentrations and the subunit stoichiometry of key protein complexes.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlejandro Brenes
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-14 01:25:13ID requested
12019-03-28 05:52:38announced
Publication List
Sinclair LV, Howden AJ, Brenes A, Spinelli L, Hukelmann JL, Macintyre AN, Liu X, Thomson S, Taylor PM, Rathmell JC, Locasale JW, Lamond AI, Cantrell DA, Antigen receptor control of methionine metabolism in T cells. Elife, 8():(2019) [pubmed]
Keyword List
submitter keyword: mouse, t cell, mtor, mtorc1, rapamycin, tmt, fusion
Contact List
Doreen Cantrell
contact affiliationCantrell Lab, Cell Signalling & Immunology, School of Life Sciences, University of Dundee
contact emaild.a.cantrell@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliationUniversity of Dundee
contact emailajbrenesmurillo@dundee.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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