PXD012058

PXD012058 is an original dataset announced via ProteomeXchange.

Title	Defining the T cell proteome and the role of mTORC1 during differentiation using quantitative proteomics
Description	High-resolution quantitative mass-spectrometry reveals similarities and disparities in how murine naïve CD4 and CD8 T cells respond to immune activation and re-structure proteomes as they differentiate to effector cells. The data map core transcriptional, metabolic and protein synthesis machinery, nutrient transporters and environment sensing molecules and reveal differences in the biosynthetic capacity of CD4 and CD8 T cells. One key nutrient sensing kinase is mammalian target of rapamycin complex1 (mTORC1). The current study identifies common and distinct mTORC1 regulated processes and divergent outcomes of mTORC1 inhibition in naïve versus effector CD4 and CD8 T cell populations. The data provide a resource that maps how immune activation and mTORC1 reshape CD4 and CD8 T cell proteomes and highlights that a deep understanding of T cell phenotype requires modelling of the impact of immune regulators on protein copy number, cellular protein concentrations and the subunit stoichiometry of key protein complexes.
HostingRepository	PRIDE
AnnounceDate	2019-03-28
AnnouncementXML	Submission_2019-03-28_05:52:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Brenes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-14 01:25:13	ID requested
⏵ 1	2019-03-28 05:52:38	announced

Sinclair LV, Howden AJ, Brenes A, Spinelli L, Hukelmann JL, Macintyre AN, Liu X, Thomson S, Taylor PM, Rathmell JC, Locasale JW, Lamond AI, Cantrell DA, Antigen receptor control of methionine metabolism in T cells. Elife, 8():(2019) [pubmed]

submitter keyword: mouse, t cell, mtor, mtorc1, rapamycin, tmt, fusion

Doreen Cantrell
contact affiliation	Cantrell Lab, Cell Signalling & Immunology, School of Life Sciences, University of Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliation	University of Dundee
contact email	ajbrenesmurillo@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD012058

PRIDE project URI

• PRIDE
  1. PXD012058
     1. Label: PRIDE project
     2. Name: Defining the T cell proteome and the role of mTORC1 during differentiation using quantitative proteomics