PXD012055

PXD012055 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins differentially regulated upon perturbed mitochondrial gene expression
Description	The aim of this project was to search for the new factors engaged in mitochondrial nucleic acids metabolism under stress conditions. To this end we treated human 293 cells with mitochondrial replication/transcription inhibitor ethidium bromide and we compared proteomes of untreated vs treated cells. We were looking for the proteins whose level would be differentially regulated in response to disturbed mitochondrial gene expression. In our studies we utilized a stable 293 cell line that inducible expresses a fusion of EGFP with mitochondria targeting sequence. This approach enabled us to control mitochondrial import by analysis of the abundance of mitochondrial EGFP marker. We isolated highly purified mitochondria from the cells, lysed them and subjected protein extracts to labelling with iTRAQ reagents. We performed quantitative analysis of stress- induced changes of human mitochondrial proteome with the use of MaxQuant software.
HostingRepository	PRIDE
AnnounceDate	2019-06-13
AnnouncementXML	Submission_2019-07-12_03:00:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Cysewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-14 01:21:02	ID requested
1	2019-06-13 07:04:24	announced
⏵ 2	2019-07-12 03:00:36	announced	Updated publication reference for PubMed record(s): 31226201.

Kotrys AV, Cysewski D, Czarnomska SD, Pietras Z, Borowski LS, Dziembowski A, Szczesny RJ, Quantitative proteomics revealed C6orf203/MTRES1 as a factor preventing stress-induced transcription deficiency in human mitochondria. Nucleic Acids Res, 47(14):7502-7517(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mitochondria, LC-MS/MS,

Roman J. Szczesny
contact affiliation	Laboratory of RNA Biology and Functional Genomics Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	rszczesny@ibb.waw.pl
lab head
Dominik Cysewski
contact affiliation	Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	dominikcysewski@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD012055

PRIDE project URI

• PRIDE
  1. PXD012055
     1. Label: PRIDE project
     2. Name: Identification of proteins differentially regulated upon perturbed mitochondrial gene expression