PXD012054

PXD012054 is an original dataset announced via ProteomeXchange.

Title	High throughput generation of a resource of the human secretome in mammalian cells
Description	The proteins secreted by human tissues (the secretome) are important for the basic understanding of human biology, but also for identification of potential targets for future diagnosis and therapy. Here, we present an annotation of all predicted secreted proteins (n=2,623) with information about their spatial distribution in the human body. A high-throughput mammalian cell factory was established to create a resource of recombinant full-length proteins. This resource was used for phenotypic assays involving β-cell dedifferentiation and for development of targeted proteomics assays. A comparison between host cells, including omics analysis, shows that many of the proteins that failed to be generated in CHO cells could be rescued in human HEK 293 cells. In conclusion, the human secretome has been mapped and characterized to facilitate further exploration of the human secretome.
HostingRepository	PanoramaPublic
AnnounceDate	2021-03-15
AnnouncementXML	Submission_2021-03-15_07:38:23.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Andreas Hober
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-13 16:36:01	ID requested
⏵ 1	2021-03-15 07:38:24	announced

Tegel H, Dannemeyer M, Kanje S, Sivertsson Å, Berling A, Svensson AS, Hober A, Enstedt H, Volk AL, Lundqvist M, Moradi M, Afshari D, Ekblad S, Xu L, Westin M, Bidad F, Schiavone LH, Davies R, Mayr LM, Knight S, G, ö, pel SO, Voldborg BG, Edfors F, Forsstr, ö, m B, von Feilitzen K, Zwahlen M, Rockberg J, Takanen JO, Uhl, é, n M, Hober S, High throughput generation of a resource of the human secretome in mammalian cells. N Biotechnol, 58():45-54(2020) [pubmed]

submitter keyword: dia, human secretome, assay development, plasma

Bjorn Forsstrom
contact affiliation	Science for Life Laboratory, KTH - Royal Institute of Technology
contact email	bjorn.forsstrom@scilifelab.se
lab head
Andreas Hober
contact affiliation	Science for Life Laboratory, KTH - Royal Institute of Technology
contact email	andreas.hober@scilifelab.se
dataset submitter

Panorama Public dataset URI