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PXD012047

PXD012047 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of Aerococcus and Globicatella spp. colonizing polymicrobial urethral catheter biofilms
DescriptionBoth Aerococcus urinae (Au) and Globicatella sanguinis (Gs) colonize the human urinary tract and are in the Aerococcaceae family. These rarely pathogenic Gram-positive bacteria were identified in polymicrobial urethral catheter biofilms (CBs) using 16S rDNA and proteomic analyses in this study. For confirming the identities, Au and Gs strains were isolated from small blood agar colonies derived from the CB extracts. Longitudinal surveys of clinical urine specimens revealed their persistence in the urinary tract and recolonization of newly replaced catheters. Dominant CB cohabitating organisms were Enterobacteriaceae, especially Proteus mirabilis and Escherichia coli. The proteomes of Gs and Au profiled from the in vivo milieu suggest that their energy metabolisms rely on glycolytic, heterolactic fermentation and peptide catabolic pathways. Several PTS sugar uptake and oligopeptide ABC transport systems were also highly abundant in the in vivo proteomes of Au and Gs, indicative to adaptations to nutrients available in urine and exfoliated urothelial cells (protein and proteoglycan breakdown products). Differences in Au and Gs metabolisms pertained to citrate lyase and glycogen (only in the Gs proteome), use of Xfp to degrade D-xylulose-5’-phosphate, and synthesis pathways for enzyme cofactors pyridoxal 6’-phosphate and 4’-phosphopantothenate (the latter only in the Au proteome). Interestingly, predicted metal ion (ZnuA-like) uptake systems were abundant in Gs but not in Au in vivo. Au expressed two LPXTG-anchored surface proteins, one predicted to have a pilin D adhesion motif. We describe how two microorganisms not previously characterized metabolically adapt to the milieu in the catheterized human urinary tract. Whether they are true pathogens or bystanders in CBs needs further investigation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:58:39.143.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYanbao Yu
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Globicatella sanguinis; NCBI TaxID: 13076; scientific name: Aerococcus urinae NBRC 15544 = CCUG 36881; NCBI TaxID: 1216979;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-13 01:47:45ID requested
12019-11-12 16:44:41announced
22024-10-22 03:58:41announced2024-10-22: Updated project metadata.
Publication List
Yu Y, Tsitrin T, Bekele S, Thovarai V, Torralba MG, Singh H, Wolcott R, Doerfert SN, Sizova MV, Epstein SS, Pieper R, Persist in Polymicrobial Urethral Catheter Biofilms Examined in Longitudinal Profiles at the Proteomic Level. Biochem Insights, 12():1178626419875089(2019) [pubmed]
10.1177/1178626419875089;
Keyword List
curator keyword: Metaproteomics, Biomedical
submitter keyword: Aerococcus, Urethral catheter biofilms, UTI, Globicatella, urinary tract infection, Proteomics
Contact List
Yanbao Yu
contact affiliationJ. Craig Venter Institute, Rockville, MD 20850
contact emailyayu@jcvi.org
lab head
Yanbao Yu
contact affiliationUniversity of Delaware
contact emailyayu@jcvi.org
dataset submitter
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Dataset FTP location
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