PXD012046

PXD012046 is an original dataset announced via ProteomeXchange.

Title	Oxidized fibrinogen HPLC-MS/MS
Description	Fibrinogen is highly susceptible to oxidation compared to other plasma proteins. Fibrinogen oxidation damages its structure and affects the protein function. Fibrinogen was isolated from citrated human plasma by the modified cold ethanol precipitation technique. Oxidation of fibrinogen by ozone: a solution of 2.0 mg of fibrinogen in 1 ml of 0.05 M Tris/0.15 M NaCl buffer (pH 7.4) was introduced into a quartz reactor filled with ozone–oxygen mixture. The full exhaustion of ozone in each experiment was confirmed by spectrophotometry by the absorption band at 254 nm. The amount of ozone was varied in the range of 50–100 μM per 1 μM of fibrinogen. Ozone-induced oxidative modifications of the fibrinogen Aα, Bβ, and γ polypeptide chains upon addition of various amounts of the oxidizer have been studied by mass-spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-04-04
AnnouncementXML	Submission_2019-04-04_00:04:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012046
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lyubov Yurina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	GG; Oxidation; Lys->AminoadipicAcid; Pro->pyro-Glu; Carboxy; Phospho; Didehydro; Deamidated; Trp->Kynurenin; Pro->Pyrrolidinone; Trp->Hydroxykynurenin; Trp->Oxolactone; Arg->GluSA; Nitro; dichlorination; Formyl; Carbamyl; Lys->Allysine; Carbamidomethyl; Gln->pyro-Glu; Trioxidation; Tyr->Dha; Amino; Glu->pyro-Glu; Dioxidation; Chlorination; Pro->Pyrrolidone
Instrument	LTQ FT Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-13 01:45:57	ID requested
⏵ 1	2019-04-04 00:04:24	announced

Yurina L, Vasilyeva A, Indeykina M, Bugrova A, Biryukova M, Kononikhin A, Nikolaev E, Rosenfeld M, Ozone-induced damage of fibrinogen molecules: identification of oxidation sites by high-resolution mass spectrometry. Free Radic Res, 53(4):430-455(2019) [pubmed]

submitter keyword: oxidation, fibrinogen, oxidative post-translational modifications, HPLC-MS/MS

Mark Rosenfeld
contact affiliation	Laboratory of biosystem thermodynamics, N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia
contact email	rosenfeld41@mail.ru
lab head
Lyubov Yurina
contact affiliation	N.M. Emanuel Institute of Biochemical Physics RAS (IBCP RAS)
contact email	mourimotonari91@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012046
     1. Label: PRIDE project
     2. Name: Oxidized fibrinogen HPLC-MS/MS