PXD012032

PXD012032 is an original dataset announced via ProteomeXchange.

Title	Identification of DHC2 associated nuclear proteins in DHC2 knockdown U87 cells
Description	The acquisition of temozolomide resistance is a major clinical challenge for glioblastoma treatment. Chemoresistance in glioblastoma is largely attributed to repair of temozolomide-induced DNA lesions by MGMT. However, many MGMT-negative glioblastomas are still resistant to temozolomide, and the underlying molecular mechanisms remain unclear. We found that DHC2 was highly expressed in MGMT-negative recurrent glioblastoma specimens and its expression strongly correlated to poor progression-free survival in MGMT-negative glioblastoma patients. In vivo and in vitro, silencing DHC2 enhanced temozolomide-induced DNA damage and significantly improved the efficiency of temozolomide treatment in MGMT-negative glioblastoma cells. It is known DHC2 is related to intracellular cargo transportation. To identify the potential interacted “cargo” DHC2 transported and explore the underlying molecular mechanisms of DHC2-midiated DNA damage repair, we performed subcellular proteomic and bioinformatic analyses.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_04:07:06.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yawei Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-12 06:47:45	ID requested
⏵ 1	2022-02-28 04:07:07	announced

Dataset with its publication pending

submitter keyword: glioblastoma, DHC2, MGMT, Label-free, subcellular proteomics

Yawei Liu
contact affiliation	Department of Neurosurgery, Nanfang hospital, Southern Medical University, Guangzhou 510515, China
contact email	liuyawei@smu.edu.cn
lab head
Yawei Liu
contact affiliation	Department of Neurosurgery, Nanfang hospital, Southern Medical University, Guangzhou 510515,China
contact email	liuyawei@smu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012032
     1. Label: PRIDE project
     2. Name: Identification of DHC2 associated nuclear proteins in DHC2 knockdown U87 cells