PXD012030

PXD012030 is an original dataset announced via ProteomeXchange.

Title	Linking the Vegetative Cell and Spore Proteomes of Peptoclostridium difficile
Description	Peptoclostridium difficile, an anaerobic pathogen, known as causative agent of pseudomembranous colitis and nosocomial diarrhoea. It can also form highly resistant endospores which lay the basic foundations of infection prior germination in the human guts. P. difficile exploits toxins TcdA and TcdB as well as a plethora of other proteins to infect the host. The present study is exclusively focused on the interconnection between vegetative cell and spore proteomes. A novel method developed for 15N metabolic labelling of P. difficile vegetative cells has enabled mass spectrometric quantification of relative protein levels of this pathogen. A total of 1095 proteins has been identified over the combined spores and vegetative cells. From this 796 proteins have been relatively and reproducibly quantified between spores and vegetative cells. Of the quantified proteins 80% are common to both the vegetative cells and spores, indicating that pathogenic P. difficile employs a relatively modest proteomic changeover for survival. Also of the identified proteins are 20%putative membrane proteins which may provide essential targets to combat P. difficile infections. Detailed data analysis has qualified potential biomarkers for diagnostic purposes.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_13:59:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wishwas R. Abhyankar
SpeciesList	scientific name: Clostridioides difficile 630; NCBI TaxID: 272563;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	apex ultra

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-12 06:04:54	ID requested
⏵ 1	2020-05-26 13:59:59	announced

Abhyankar WR, Zheng L, Brul S, de Koster CG, de Koning LJ, Show Finite Differences and Reveal Potential Protein Markers. J Proteome Res, 18(11):3967-3976(2019) [pubmed]

submitter keyword: Peptoclostridium difficile, spores, one-pot sample processing, proteomics

Chris G. de Koster
contact affiliation	Department of Mass Spectrometry of Biomacromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands
contact email	C.G.deKoster@uva.nl
lab head
Wishwas R. Abhyankar
contact affiliation	University of Amsterdam
contact email	w.r.abhyankar@uva.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD012030

PRIDE project URI

• PRIDE
  1. PXD012030
     1. Label: PRIDE project
     2. Name: Linking the Vegetative Cell and Spore Proteomes of Peptoclostridium difficile