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PXD012026

PXD012026 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemical phosphoproteomic approaches reveal substrate specificity of protein phosphatases-1 and -2
DescriptionThe phosphatases PP1 and PP2A are responsible for the majority of dephosphorylation reactions on phosphoserine (pS) and –threonine (pT), and are involved in basically all cellular processes and many related diseases. They are thought to have no appreciable intrinsic substrate specificity, but to gain specificity only in their holoenzyme forms. Through the development of a peptide library approach and application of a complementary phosphoproteomics assay, we uncover that PP1 and PP2A show intrinsic specificity towards pT over pS, as well as toward the sequence context surrounding the phospho-site. Our substrate specificity data reveal that PP1 is a key regulator of the 14-3-3 protein binding motif, which enabled us to establish an unknown role for PP1 as a regulator of the GRB-associated-binding-protein 2 downstream (Gab2)/14-3-3 complex, exemplifying predictive potential of the data. Thus, this data should serve as a rich resource for (de)phosphorylation studies covering multiple cellular processes and phosphoproteins.
HostingRepositoryPRIDE
AnnounceDate2020-07-14
AnnouncementXMLSubmission_2020-08-11_04:33:33.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephanie Heinzlmeir
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-12 00:20:48ID requested
12020-07-14 03:49:31announced
22020-08-11 04:33:34announced2020-08-11: Updated publication reference for PubMed record(s): 32681005.
Publication List
Hoermann B, Kokot T, Helm D, Heinzlmeir S, Chojnacki JE, Schubert T, Ludwig C, Berteotti A, Kurzawa N, Kuster B, Savitski MM, K, ö, hn M, Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A. Nat Commun, 11(1):3583(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: phosphatase substrates, co-immunoprecipitation, quantitative phosphoproteomics
Contact List
BayBioMS Bavarian Center for Biomolecular Mass Spectrometry
contact affiliationTechnical University of Munich TUM School of Life Scienes Weihenstephan Freising, Germany
contact emailstephanie.heinzlmeir@tum.de
lab head
Stephanie Heinzlmeir
contact affiliationChair of Proteomics and Bioanalytics, Technische Universität München, Germany
contact emailstephanie.heinzlmeir@tum.de
dataset submitter
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Dataset FTP location
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