PXD012025

PXD012025 is an original dataset announced via ProteomeXchange.

Title	Drosophila nephrocyte proteome
Description	Podocyte injury is a major cause of chronic kidney disease, but the involved signaling pathways are largely unknown. Here, we used ultrasensitive proteomics to investigate the response of the native renal podocyte to injury and prioritized candidates by aligning proteinuria in individual mice with protein expression data. The analysis revealed large perturbations of signaling pathways known to associate with FSGS, but also showed perturbation in metabolism and proteins responding to mechanical stress. We employed Drosophila nephrocytes for further functional analysis of selected candidates and studied filtration function and endocytosis. Among the candidates identified was the mechanosensor protein Filamin-B which is a central component of a conserved cellular response to mechanical stress in podocytes in vitro. Loss of Cher, the orthologue of Filamin-B, led to an increased endocytosis in nephrocytes. Of note, Filamin-B expression was only elevated in the Doxorubicin-induced proteome of podocytes but not after LPS induced injury despite the regulation of multiple similar pathways on the systems level. The Filamin-B amount in glomeruli of proteinuric PAN rats correlated with the amount of albumin . In conclusion, this study identified conserved mechanisms of podocyte stress response employing state of the art ultrasensitive proteomics in isolated mouse podocytes and Drosophila nephrocytes.
HostingRepository	PRIDE
AnnounceDate	2020-07-29
AnnouncementXML	Submission_2020-07-29_04:14:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Rinschen
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-12 00:18:23	ID requested
⏵ 1	2020-07-29 04:14:46	announced

Koehler S, Kuczkowski A, Kuehne L, J, ü, ngst C, Hoehne M, Grahammer F, Eddy S, Kretzler M, Beck BB, H, ö, hfeld J, Schermer B, Benzing T, Brinkkoetter PT, Rinschen MM, Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis. J Am Soc Nephrol, 31(3):544-559(2020) [pubmed]

curator keyword: Biological

submitter keyword: Drosophila, nephrocyte

Markus Rinschen
contact affiliation	University Hospital Cologne
contact email	markus.rinschen@uk-koeln.de
lab head
Markus Rinschen
contact affiliation	Kidney research center cologne
contact email	markus.rinschen@uk-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD012025

PRIDE project URI

• PRIDE
  1. PXD012025
     1. Label: PRIDE project
     2. Name: Drosophila nephrocyte proteome