PXD012017

PXD012017 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic analysis of the NF2/Merlin Interactome by Proximity Biotinylation
Description	Neurofibromatosis type 2 is an inherited neoplastic disease consisting of schwannomas, meningiomas, and ependymomas that is caused by inactivation of the tumor suppressor gene NF2. The NF2 gene product, merlin, has no intrinsic catalytic activity; its tumor suppressor function is mediated through the proteins with which it interacts. However, there is no consensus about which merlin interactions are necessary for tumor suppression. We used proximity biotinylation followed by mass spectrometry and direct binding assays to characterize the proteins that associate with merlin and merlin mutants in immortalized Schwann cells. We identified 52 proteins that associate with merlin, including a previously unreported merlin binding protein, ASPP2. Our results identify merlin as a component of mechanosensing signal transduction pathways in cell junctions, in the context of a specific set of structures and molecules through which it acts, in a cell type relevant to schwannoma formation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:35.885.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Hennigan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 08:46:27	ID requested
1	2019-04-25 00:20:08	announced
⏵ 2	2024-10-22 03:58:44	announced	2024-10-22: Updated project metadata.

Publication List

Hennigan RF, Fletcher JS, Guard S, Ratner N, Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal, 12(578):(2019) [pubmed]
10.1126/scisignal.aau8749;

Hennigan RF, Fletcher JS, Guard S, Ratner N, Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal, 12(578):(2019) [pubmed]

10.1126/scisignal.aau8749;

Keyword List

submitter keyword: Proximity Biotinylation, Schwann cell., NF2,Neurofibromatosis Type 2, Merlin, Interactome, BioIB

submitter keyword: Proximity Biotinylation, Schwann cell., NF2,Neurofibromatosis Type 2, Merlin, Interactome, BioIB

Contact List

Nancy Ratner
contact affiliation	Experimental Hematology and Cancer Biology Cincinnati Children's Hopital Medical Center
contact email	Nancy.Ratner@cchmc.org
lab head
Robert Hennigan
contact affiliation	Cincinnati Children's Hospital Medical Center
contact email	robert.hennigan@cchmc.org
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD012017

PRIDE project URI

Repository Record List

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