PXD012013

PXD012013 is an original dataset announced via ProteomeXchange.

Title	Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids
Description	Methyl ketone production by P. putida with A. thaliana and switchgrass hydrolysates obtained by dilute acid pretreatment led to the identification of plant-derived amino acids, rather than mono-aromatics, as key stimulative components of these hydrolysates. Shotgun proteomics indicated that the amino acids had a specific inductive effect on proteins involved in fatty acid biosynthesis, leading to a 9-fold increase in methyl ketone titer when amending glucose-containing minimal medium with a defined set of amino acids.
HostingRepository	PRIDE
AnnounceDate	2019-06-27
AnnouncementXML	Submission_2019-06-27_00:40:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD012013
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Petzold
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 05:18:42	ID requested
⏵ 1	2019-06-27 00:40:32	announced

Dong J, Chen Y, Benites VT, Baidoo EEK, Petzold CJ, Beller HR, Eudes A, Scheller HV, Adams PD, Mukhopadhyay A, Simmons BA, Singer SW, Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids. Biotechnol Bioeng, 116(8):1909-1922(2019) [pubmed]

curator keyword: Biological

submitter keyword: lignin-related aromatics

methyl ketones

biomass hydrolysates

protein

amino acids.

Christopher J. Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012013
     1. Label: PRIDE project
     2. Name: Methyl ketone production by Pseudomonas putida is enhanced by plant-derived amino acids