PXD012010

PXD012010 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of cyclic stretch A549 and IMR-90
Description	Lung cancer is one of the leading causes of death. However, most of the researches were based on the traditional cell-culturing method. Whereas cells of lung are subjected to the mechanical forces periodically while breathing. In the present study, we applied cyclic stretch to stimulate the continuously contracting physical condition. We uncovered the stretching force-induced phosphoproteome in lung cancer cell A549 and fibroblast IMR-90. 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins were identified in A549 and IMR-90, respectively. Interestingly, cytoskeleton reorganization and mitochondrial localization were enriched in the significantly expressed phosphoproteins in response to cyclic stretch. Indeed, we found this physical stress changed cell alignment thus disrupted mitochondrial dynamics. We proved that mitochondrial fusion is induced by uniaxial stretch in 2 cell lines. This study reveals the molecular mechanism of cyclic stretch and supports that stretching force enhanced cellular rearrangement and mitochondrial fusion in lung cells.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_08:08:03.108.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wang Weihsuan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 02:08:31	ID requested
⏵ 1	2021-09-08 08:08:03	announced

Wang WH, Hsu CL, Huang HC, Juan HF, Quantitative Phosphoproteomics Reveals Cell Alignment and Mitochondrial Length Change under Cyclic Stretching in Lung Cells. Int J Mol Sci, 21(11):(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Phosphoproteome

cyclic stretch

Lund cancer

Lung fibroblast

Hseuh-Fen Juan
contact affiliation	Genome and Systems Biology Degree Program, National Taiwan University, Department of Life Science, National Taiwan University, Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University,
contact email	yukijuan@ntu.edu.tw
lab head
Wang Weihsuan
contact affiliation	National Taiwan University
contact email	weihsuan15@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012010
     1. Label: PRIDE project
     2. Name: Phosphoproteome of cyclic stretch A549 and IMR-90