PXD012008

PXD012008 is an original dataset announced via ProteomeXchange.

Title	Pentatricopeptide repeat poly(A) binding protein from mitochondria of trypanosomes
Description	In Trypanosoma brucei, most mitochondrial mRNAs undergo U-insertion/deletion editing, and 3′ adenylation and uridylation. The internal sequence changes and terminal extensions are coordinated: Pre-editing addition of the short (A) tail protects the edited transcript against 3′-5′ degradation, while post-editing A/U-tailing renders mRNA competent for ribosome recruitment. Participation of a poly(A) binding protein (PABP) in coupling of editing and 3′ modification processes has been inferred, but its identity and mechanism of action remained elusive. We report identification of KPAF4, a pentatricopeptide repeat-containing PABP which sequesters the A-tail and impedes exonucleolytic degradation. Conversely, KPAF4 inhibits uridylation of A-tailed transcripts and, therefore, premature A/U-tailing of partially-edited mRNAs. This quality check point prevents translation of incompletely edited mRNAs. Our findings also implicate the RNA editing substrate binding complex (RESC) in mediating the interaction between the 5′-end bound pyrophosphohydrolase MERS1 and 3′-end associated KPAF4 to enable mRNA circularization. This event is critical for transcript stability during the editing process.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:33.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clinton Yu
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 01:54:57	ID requested
1	2019-04-02 07:18:34	announced
⏵ 2	2024-10-22 03:58:41	announced	2024-10-22: Updated project metadata.

Mesitov MV, Yu T, Suematsu T, Sement FM, Zhang L, Yu C, Huang L, Aphasizheva I, Pentatricopeptide repeat poly(A) binding protein KPAF4 stabilizes mitochondrial mRNAs in Trypanosoma brucei. Nat Commun, 10(1):146(2019) [pubmed]

10.1038/s41467-018-08137-2;

curator keyword: Biological

submitter keyword: PPR protein,Trypanosoma, RNA stability, mitochondria, RNA editing, polyadenylation

Lan Huang
contact affiliation	University of California, Irvine
contact email	lanhuang@uci.edu
lab head
Clinton Yu
contact affiliation	University of California, Irvine
contact email	clinton.yu@uci.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD012008
     1. Label: PRIDE project
     2. Name: Pentatricopeptide repeat poly(A) binding protein from mitochondria of trypanosomes