PXD012005

PXD012005 is an original dataset announced via ProteomeXchange.

Title	Polypeptide targeting to distinct areas of the human ER
Description	There is at least one additional targeting pathway for proteins to the human endoplasmic reticulum, which involves a cytosolic as well as an ER-membrane resident receptor, belonging to the family of PEX proteins. In contrast to the precursor protein targeting pathways, the additional pathway targets proteins to distinct ER sites and does not target proteins to the Sec61 complex. To characterize substrate proteins of this pathway, we combined siRNA-mediated PEX3-depletion in HeLa cells with label-free quantitative proteomics and and differential protein abundance analysis.
HostingRepository	PRIDE
AnnounceDate	2021-12-20
AnnouncementXML	Submission_2021-12-20_08:37:07.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Zimmermann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-11 00:10:35	ID requested
⏵ 1	2021-12-20 08:37:08	announced

Zimmermann R, Lang S, Lerner M, F, ö, rster F, Nguyen D, Helms V, Schrul B, Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER. Int J Mol Sci, 22(23):(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: HeLa, PEX3

Prof. Dr. Bianca Schrul & Prof. Dr. Richard Zimmermann (lab heads)
contact affiliation	Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Kirrbergerstr. 100, 66421 Homburg, Deutschland Dr. Nagarjuna Nagaraj, Max-Planck Institute for Biochemistry, Core facility (contact)
contact email	richard.zimmermann@uks.eu
lab head
Richard Zimmermann
contact affiliation	Saarland University
contact email	richard.zimmermann@uks.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD012005

PRIDE project URI

• PRIDE
  1. PXD012005
     1. Label: PRIDE project
     2. Name: Polypeptide targeting to distinct areas of the human ER