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PXD012004

PXD012004 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePolvi_yeastfilamentation_P50_VS12_SAINT3869_5600
DescriptionMorphogenetic transitions are prevalent in the fungal kingdom. For a leading human fungal pathogen, Candida albicans, the capacity to transition between yeast and filaments is key for virulence. For the model yeast Saccharomyces cerevisiae, filamentation enables nutrient acquisition. A recent functional genomic screen in S. cerevisiae identified Mfg1 as a regulator of morphogenesis that acts in complex with Flo8 and Mss11 to mediate transcriptional responses crucial for filamentation. In C. albicans, Mfg1 also interacts physically with Flo8 and Mss11 and is critical for filamentation in response to diverse cues, but the mechanisms through which it regulates morphogenesis remained elusive. Here, we explored the consequences of perturbation of Mfg1, Flo8, and Mss11 on C. albicans morphogenesis, and identified functional divergence of complex members. We observed that C. albicans Mss11 was dispensable for filamentation, and that overexpression of FLO8 caused constitutive filamentation even in the absence of Mfg1. Harnessing transcriptional profiling and chromatin immunoprecipitation coupled to microarray analysis, we identified divergence between transcriptional targets of Flo8 and Mfg1 in C. albicans. We also established that Flo8 and Mfg1 cooperatively bind to promoters of key regulators of filamentation, including TEC1, for which overexpression was sufficient to restore filamentation in the absence of Flo8 or Mfg1. To further explore the circuitry through which Mfg1 regulates morphogenesis, we employed a novel strategy to select for mutations that restore filamentation in the absence of Mfg1. Whole genome sequencing of filamentation-competent mutants revealed chromosome 6 amplification as a conserved adaptive mechanism. A key determinant of the chromosome 6 amplification is FLO8, as deletion of one allele blocked morphogenesis, and chromosome 6 was not amplified in evolved lineages for which FLO8 was re-located to a different chromosome. Thus, this work highlights rewiring of key morphogenetic regulators over evolutionary time and aneuploidy as an adaptive mechanism driving fungal morphogenesis.
HostingRepositoryMassIVE
AnnounceDate2018-12-10
AnnouncementXMLSubmission_2018-12-10_10:25:50.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGingras Lab
SpeciesList scientific name: Candida albicans; NCBI TaxID: 5476;
ModificationListdeamidated L-asparagine; deamidated L-glutamine; L-methionine sulfoxide
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-10 10:11:20ID requested
12018-12-10 10:25:51announced
Publication List
no publication
Keyword List
submitter keyword: Affinity purification, Protein-protein interaction, AP-MS, Mfg1, Yeast filamentation
Contact List
Anne-Claude Gingras
contact affiliationLTRI
contact emailgingras@lunenfeld.ca
lab head
Gingras Lab
contact affiliationSamuel Lunenfeld Research Institute
contact emailgingras@mshri.on.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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