PXD012003 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An insight into the proteome of uveal melanoma-derived ectosomes reveals the presence of potentially useful biomarkers |
Description | Cancer cells are known to release extracellular vesicles that often promote disease development and progression. The present study investigated protein content and glycosylation pattern of ectosomes released in vitro by human primary uveal melanoma Mel202 cell line. Ectosomes released by Mel202 cells were isolated from conditioned media by sequential centrifugation and nanoLC-MS/MS approach was used to determine their protein content. Subsequently, proteins from ectosomes, the whole cell extracts and the membrane fractions were probed with a panel of lectins to reveal characteristic glycan structures. As many as 2529 unique proteins were identified, and many of them are known to be involved in cancer cell proliferation and altered metabolism, tumor invasion, metastasis or drug resistance. Lectin-based studies showed a distinct glycosylation pattern between Mel202 ectosomes and parental cell membranes. Selective enrichment of ectosomal proteins with bisected complex type N-glycans and α2,6-linked sialic acid may be significant for ectosome formation and sequestration. Our data supports recent findings that ectosomes derive from particular regions of the cell membrane and thus contain a unique protein and glycan composition. Exploring whether cancer-related proteins and glycans are enriched in ectosomes isolated from body fluids of cancer patients should became a subject of future in-depth studies. |
HostingRepository | PRIDE |
AnnounceDate | 2019-08-08 |
AnnouncementXML | Submission_2019-08-08_05:59:59.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Urszula Jankowska |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-10 08:28:34 | ID requested | |
⏵ 1 | 2019-08-08 06:00:00 | announced | |
Publication List
Keyword List
curator keyword: Biomedical |
submitter keyword: ectosomes, extracellular vesicles, glycosylation, lectins, proteomics, uveal melanoma, |
Contact List
Sylwia Kedracka-Krok |
contact affiliation | Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland Laboratory of Proteomics and Mass Spectrometry, Malopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland |
contact email | sylwia.kedracka-krok@uj.edu.pl |
lab head | |
Urszula Jankowska |
contact affiliation | Proteomics and Mass Spectrometry Laboratory, Malopolska Centre of Biotechnology, Jagiellonian University |
contact email | urszula.jankowska@uj.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD012003 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD012003
- Label: PRIDE project
- Name: An insight into the proteome of uveal melanoma-derived ectosomes reveals the presence of potentially useful biomarkers